IRIS PubliCatt
PubliCatt è il repository istituzionale ad accesso aperto dell'Università Cattolica del Sacro Cuore, dove gli utenti autorizzati afferenti all'Ateneo provvedono direttamente e autonomamente a depositare e a rendere visibili le proprie pubblicazioni, inserendo i dati descrittivi del documento stesso ("metadati", quali il titolo, autore, abstract, etc.) e, laddove possibile, il testo della pubblicazione stessa (full-text).
PubliCatt utilizza la piattaforma IRIS (Institutional Research Information System) sviluppata da CINECA.
EnglishFumonisins B (FBs) can occur in maize as free forms and as hidden FBs (interaction between FBs and matrix macro-constituents). The analytical methods to detect free FBs in maize and derived products usually involve extraction with aqueous methanol and/or acetonitrile; for hidden FBs, alkaline hydrolysis (2MKOH) and following CH3CN extraction is used. Recently, we proposed a simple phosphate buffer (PB) extraction to detect free FBs in masa and maize samples. This study aimed at evaluating if this method is suitable to detect also hidden FBs; then, a comparison between the most common solvents, PB, and alkaline hydrolysis was carried out. For maize samples, the results showed that 0.4 M PB can extract both free and most hidden FBs; however, this method was not suitable for some categories of maize-based processed food products, in particular cornflakes, chips, and crispbreads. To solve this drawback, a modified PB extraction, which entails the addition of Carrez solutions in order to precipitate proteins, was tested and satisfactory results were obtained. Finally, the alkaline hydrolysis of maize-based food extracts showed that bound FBs can be co-extracted together with free forms by 0.4 M PB and quantified as hydrolyzed FBs.
Bertuzzi, T., Mulazzi, A., Rastelli, S., Pietri, A., Hidden Fumonisins: Simple and Innovative Extractions for Their Determination in Maize and Derived Products, <<FOOD ANALYTICAL METHODS>>, 2016; 2016 (9): 1970-1979. [doi:DOI 10.1007/s12161-015-0377-2] [http://hdl.handle.net/10807/78145]
| Autori: | ||
| Titolo: | Hidden Fumonisins: Simple and Innovative Extractions for Their Determination in Maize and Derived Products | |
| Digital Object Identifier (DOI): | http://dx.doi.org/DOI 10.1007/s12161-015-0377-2 | |
| Data di pubblicazione: | 2016 | |
| Abstract: | Fumonisins B (FBs) can occur in maize as free forms and as hidden FBs (interaction between FBs and matrix macro-constituents). The analytical methods to detect free FBs in maize and derived products usually involve extraction with aqueous methanol and/or acetonitrile; for hidden FBs, alkaline hydrolysis (2MKOH) and following CH3CN extraction is used. Recently, we proposed a simple phosphate buffer (PB) extraction to detect free FBs in masa and maize samples. This study aimed at evaluating if this method is suitable to detect also hidden FBs; then, a comparison between the most common solvents, PB, and alkaline hydrolysis was carried out. For maize samples, the results showed that 0.4 M PB can extract both free and most hidden FBs; however, this method was not suitable for some categories of maize-based processed food products, in particular cornflakes, chips, and crispbreads. To solve this drawback, a modified PB extraction, which entails the addition of Carrez solutions in order to precipitate proteins, was tested and satisfactory results were obtained. Finally, the alkaline hydrolysis of maize-based food extracts showed that bound FBs can be co-extracted together with free forms by 0.4 M PB and quantified as hydrolyzed FBs. | |
| Lingua: | Inglese | |
| Rivista: | ||
| Citazione: | Bertuzzi, T., Mulazzi, A., Rastelli, S., Pietri, A., Hidden Fumonisins: Simple and Innovative Extractions for Their Determination in Maize and Derived Products, <<FOOD ANALYTICAL METHODS>>, 2016; 2016 (9): 1970-1979. [doi:DOI 10.1007/s12161-015-0377-2] [http://hdl.handle.net/10807/78145] | |
| Appare nelle tipologie: | Articolo in rivista, Nota a sentenza |
File in questo prodotto:
http://hdl.handle.net/10807/78145
Copyright © 2021