Fumonisins B (FBs) can occur in maize as free forms and as hidden FBs (interaction between FBs and matrix macro-constituents). The analytical methods to detect free FBs in maize and derived products usually involve extraction with aqueous methanol and/or acetonitrile; for hidden FBs, alkaline hydrolysis (2MKOH) and following CH3CN extraction is used. Recently, we proposed a simple phosphate buffer (PB) extraction to detect free FBs in masa and maize samples. This study aimed at evaluating if this method is suitable to detect also hidden FBs; then, a comparison between the most common solvents, PB, and alkaline hydrolysis was carried out. For maize samples, the results showed that 0.4 M PB can extract both free and most hidden FBs; however, this method was not suitable for some categories of maize-based processed food products, in particular cornflakes, chips, and crispbreads. To solve this drawback, a modified PB extraction, which entails the addition of Carrez solutions in order to precipitate proteins, was tested and satisfactory results were obtained. Finally, the alkaline hydrolysis of maize-based food extracts showed that bound FBs can be co-extracted together with free forms by 0.4 M PB and quantified as hydrolyzed FBs.

Bertuzzi, T., Mulazzi, A., Rastelli, S., Pietri, A., Hidden Fumonisins: Simple and Innovative Extractions for Their Determination in Maize and Derived Products, <<FOOD ANALYTICAL METHODS>>, 2016; 2016 (9): 1970-1979. [doi:DOI 10.1007/s12161-015-0377-2] [http://hdl.handle.net/10807/78145]

Hidden Fumonisins: Simple and Innovative Extractions for Their Determination in Maize and Derived Products

Bertuzzi, Terenzio
Primo
;
Mulazzi, Annalisa
Secondo
;
Rastelli, Silvia
Penultimo
;
Pietri, Amedeo
Ultimo
2016

Abstract

Fumonisins B (FBs) can occur in maize as free forms and as hidden FBs (interaction between FBs and matrix macro-constituents). The analytical methods to detect free FBs in maize and derived products usually involve extraction with aqueous methanol and/or acetonitrile; for hidden FBs, alkaline hydrolysis (2MKOH) and following CH3CN extraction is used. Recently, we proposed a simple phosphate buffer (PB) extraction to detect free FBs in masa and maize samples. This study aimed at evaluating if this method is suitable to detect also hidden FBs; then, a comparison between the most common solvents, PB, and alkaline hydrolysis was carried out. For maize samples, the results showed that 0.4 M PB can extract both free and most hidden FBs; however, this method was not suitable for some categories of maize-based processed food products, in particular cornflakes, chips, and crispbreads. To solve this drawback, a modified PB extraction, which entails the addition of Carrez solutions in order to precipitate proteins, was tested and satisfactory results were obtained. Finally, the alkaline hydrolysis of maize-based food extracts showed that bound FBs can be co-extracted together with free forms by 0.4 M PB and quantified as hydrolyzed FBs.
Inglese
Bertuzzi, T., Mulazzi, A., Rastelli, S., Pietri, A., Hidden Fumonisins: Simple and Innovative Extractions for Their Determination in Maize and Derived Products, <<FOOD ANALYTICAL METHODS>>, 2016; 2016 (9): 1970-1979. [doi:DOI 10.1007/s12161-015-0377-2] [http://hdl.handle.net/10807/78145]
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