Natural casings derived from different intestine portions have been used for centuries in the production of fresh and dry-fermented sausages. Here we analysed by means of culture-dependent methods and Illumina high-throughput sequencing of 16S rRNA amplicons the bacterial ecology of hog, cow and ovine casings at different stages of their preparation for sausages production. Several strains of Staphylococcus, Lactobacillus, Bifidobacterium, Vagococcus and Clostridium were counted, isolated and characterised at phylogenetic level. High-throughput sequencing analyses revealed a high bacterial diversity, which differed strongly between casings of different animal species. The technological processes involved in the preparation for casing had also a strong impact on the casings bacterial ecology, with a significant reduction of undesired microorganisms, and an increase in the proportion of lactobacilli and staphylococci. Natural casings were demonstrated to be complex ecological environments, whose role as microbiological inoculants in the production of sausages should not be underestimated.

Rebecchi, A., Pisacane, V., Miragoli, F., Polka, J. U., Falasconi, I., Morelli, L., Puglisi, E., High-throughput asssessment of bacterial ecology in hog, cow and ovine casings used in sausages production, <<INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY>>, 2015; (212): 49-59 [http://hdl.handle.net/10807/68859]

High-throughput asssessment of bacterial ecology in hog, cow and ovine casings used in sausages production

Rebecchi, Annalisa;Miragoli, Francesco;Polka, Justyna Urszula;Falasconi, Irene;Morelli, Lorenzo;Puglisi, Edoardo
2015

Abstract

Natural casings derived from different intestine portions have been used for centuries in the production of fresh and dry-fermented sausages. Here we analysed by means of culture-dependent methods and Illumina high-throughput sequencing of 16S rRNA amplicons the bacterial ecology of hog, cow and ovine casings at different stages of their preparation for sausages production. Several strains of Staphylococcus, Lactobacillus, Bifidobacterium, Vagococcus and Clostridium were counted, isolated and characterised at phylogenetic level. High-throughput sequencing analyses revealed a high bacterial diversity, which differed strongly between casings of different animal species. The technological processes involved in the preparation for casing had also a strong impact on the casings bacterial ecology, with a significant reduction of undesired microorganisms, and an increase in the proportion of lactobacilli and staphylococci. Natural casings were demonstrated to be complex ecological environments, whose role as microbiological inoculants in the production of sausages should not be underestimated.
Inglese
Rebecchi, A., Pisacane, V., Miragoli, F., Polka, J. U., Falasconi, I., Morelli, L., Puglisi, E., High-throughput asssessment of bacterial ecology in hog, cow and ovine casings used in sausages production, <<INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY>>, 2015; (212): 49-59 [http://hdl.handle.net/10807/68859]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/68859
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