Three different processes were investigated for the recovery of fermentable sugars from grape stalks: autohydrolysis at 121°C before and after a pre-washing step and acid hydrolysis (2% H2SO4w/w) after a pre-washing step. Moreover, optimal conditions of a charcoal-based purification process were determined by experimental design. All hydrolysates, with their corresponding synthetic liquors were used as fermentation substrates for the production of metabolites by the yeast: Debaryomyces nepalensis NCYC 1026. The main fermentation product was ethanol, where a maximum production of 20.84g/l, a conversion yield of 0.35g ethanol/g monomeric sugars and a productivity of 0.453g/lh were obtained from non-purified autohydrolysate liquor. In all cases, ethanol production and cell growth were better in non-purified liquors than in synthetic liquors. These results could be influenced by the presence of other sugars in the hydrolysates, with higher concentration in non-purified ones.
Egues, I., Serrano, L., Amendola, D., De Faveri, D. M., Spigno, G., Labidi, J., Fermentable sugars recovery from grape stalks for bioethanol production, <<RENEWABLE ENERGY>>, 2013; 60 (Dicembre): 553-558. [doi:10.1016/j.renene.2013.06.006] [http://hdl.handle.net/10807/51348]
Fermentable sugars recovery from grape stalks for bioethanol production
Amendola, Danila;De Faveri, Dante Marco;Spigno, Giorgia;
2013
Abstract
Three different processes were investigated for the recovery of fermentable sugars from grape stalks: autohydrolysis at 121°C before and after a pre-washing step and acid hydrolysis (2% H2SO4w/w) after a pre-washing step. Moreover, optimal conditions of a charcoal-based purification process were determined by experimental design. All hydrolysates, with their corresponding synthetic liquors were used as fermentation substrates for the production of metabolites by the yeast: Debaryomyces nepalensis NCYC 1026. The main fermentation product was ethanol, where a maximum production of 20.84g/l, a conversion yield of 0.35g ethanol/g monomeric sugars and a productivity of 0.453g/lh were obtained from non-purified autohydrolysate liquor. In all cases, ethanol production and cell growth were better in non-purified liquors than in synthetic liquors. These results could be influenced by the presence of other sugars in the hydrolysates, with higher concentration in non-purified ones.
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