The purpose of this study was to assess the antimicrobial resistance profiles of several strains of Lactobacillus and Bifidobacterium spp. of probiotic interest. The International Organization for Standardization method was used to determine the minimal inhibitory concentrations of the antibiotics recommended by the European Food Safety Authority (EFSA). As a result, some of the tested microorganisms showed a visible growth up to the microbiological cutoff values indicated by EFSA guidelines in 2012. We were not able to categorize these strains as susceptible or resistant on the basis of antimicrobial resistance phenotypic testing as EFSA document does not explicitly deal with such a phenotypic condition where strains grow at antibiotic concentrations up to the established cutoff value. Although a few strains have been analyzed for this study, our findings highlight a potential challenge in accurately determining the antibiotic resistance in specific strains relevant for human and animal health.

Polka, J. U., Morelli, L., Patrone, V., Microbiological Cutoff Values: A Critical Issue in Phenotypic Antibiotic Resistance Assessment of Lactobacilli and Bifidobacteria, <<MICROBIAL DRUG RESISTANCE>>, 2016; 22 (8): 696-699. [doi:10.1089/mdr.2015.0328] [http://hdl.handle.net/10807/91354]

Microbiological Cutoff Values: A Critical Issue in Phenotypic Antibiotic Resistance Assessment of Lactobacilli and Bifidobacteria

Polka, Justyna Urszula
Primo
;
Morelli, Lorenzo
Secondo
;
Patrone, Vania
Ultimo
2016

Abstract

The purpose of this study was to assess the antimicrobial resistance profiles of several strains of Lactobacillus and Bifidobacterium spp. of probiotic interest. The International Organization for Standardization method was used to determine the minimal inhibitory concentrations of the antibiotics recommended by the European Food Safety Authority (EFSA). As a result, some of the tested microorganisms showed a visible growth up to the microbiological cutoff values indicated by EFSA guidelines in 2012. We were not able to categorize these strains as susceptible or resistant on the basis of antimicrobial resistance phenotypic testing as EFSA document does not explicitly deal with such a phenotypic condition where strains grow at antibiotic concentrations up to the established cutoff value. Although a few strains have been analyzed for this study, our findings highlight a potential challenge in accurately determining the antibiotic resistance in specific strains relevant for human and animal health.
Inglese
Polka, J. U., Morelli, L., Patrone, V., Microbiological Cutoff Values: A Critical Issue in Phenotypic Antibiotic Resistance Assessment of Lactobacilli and Bifidobacteria, <<MICROBIAL DRUG RESISTANCE>>, 2016; 22 (8): 696-699. [doi:10.1089/mdr.2015.0328] [http://hdl.handle.net/10807/91354]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/91354
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