To identify potential markers of aging, we compared the expression of a set of genes involved in mitochondrial activity in heart, skeletal muscle and brain of young and aged mice. By RT-qPCR, we analysed the transcription of cyclooxygenase-1 (COX-1), cytochrome-b (Cytb), peroxisome proliferator–activated-receptor-gamma-coactivator-1α (PGC-1α), sirt-1, sirt-2, sirt-3, sirt-6, manganese superoxide dismutase (MnSOD), UnCoupling Proteins-2 (UCP2), F3-contactin (F3-con), Ranbp17 and TNF-α in two groups of 24, 4-months-old, and 7, 22-month-old, mice. Furthermore, we analysed the relative amount of mitochondrial genome in the same tissues by qPCR. Transcription of many of these genes shows significant variations between young and old, in a tissue-specific way. While most of our observations are in agreement with the literature, in hearth, unexpectedly, even though the transcriptional activity of most genes is significantly decreased, the copy number of mitochondrial genome is noticeably increased. The meaning of this finding is worth further investigation. Blood analyses indicate an inflammatory and oxidative status in old vs. young displayed by higher concentration of haptoglobin and ROMt and lower concentration of SOD.

Dilda, F., Minuti, A., Patrone, V., Callegari, M. L., Prandini, A., Trevisi, E., Ajmone Marsan, P., Lucchini, F., Mitochondrial genome copy number increases in the hearth of aged mice, Abstract de <<7° world congress on Targeting Mitochondria>>, (Berlino, 24-26 October 2016 ), <<JOURNAL OF WORLD MITOCHONDRIA SOCIETY>>, 2016; 2 (2): 103-103. 10.18143/JWMS_v2i2 [http://hdl.handle.net/10807/86532]

Mitochondrial genome copy number increases in the hearth of aged mice

Dilda, Francesca
Primo
;
Minuti, Andrea
Secondo
;
Patrone, Vania;Callegari, Maria Luisa;Prandini, Aldo;Trevisi, Erminio;Ajmone Marsan, Paolo
Penultimo
;
Lucchini, Franco
Ultimo
2016

Abstract

To identify potential markers of aging, we compared the expression of a set of genes involved in mitochondrial activity in heart, skeletal muscle and brain of young and aged mice. By RT-qPCR, we analysed the transcription of cyclooxygenase-1 (COX-1), cytochrome-b (Cytb), peroxisome proliferator–activated-receptor-gamma-coactivator-1α (PGC-1α), sirt-1, sirt-2, sirt-3, sirt-6, manganese superoxide dismutase (MnSOD), UnCoupling Proteins-2 (UCP2), F3-contactin (F3-con), Ranbp17 and TNF-α in two groups of 24, 4-months-old, and 7, 22-month-old, mice. Furthermore, we analysed the relative amount of mitochondrial genome in the same tissues by qPCR. Transcription of many of these genes shows significant variations between young and old, in a tissue-specific way. While most of our observations are in agreement with the literature, in hearth, unexpectedly, even though the transcriptional activity of most genes is significantly decreased, the copy number of mitochondrial genome is noticeably increased. The meaning of this finding is worth further investigation. Blood analyses indicate an inflammatory and oxidative status in old vs. young displayed by higher concentration of haptoglobin and ROMt and lower concentration of SOD.
Inglese
Dilda, F., Minuti, A., Patrone, V., Callegari, M. L., Prandini, A., Trevisi, E., Ajmone Marsan, P., Lucchini, F., Mitochondrial genome copy number increases in the hearth of aged mice, Abstract de <<7° world congress on Targeting Mitochondria>>, (Berlino, 24-26 October 2016 ), <<JOURNAL OF WORLD MITOCHONDRIA SOCIETY>>, 2016; 2 (2): 103-103. 10.18143/JWMS_v2i2 [http://hdl.handle.net/10807/86532]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10807/86532
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