OBJECTIVE: To investigate the effects of N-acetylcysteine (NAC) and high-dose atorvastatin (ATOR) in reducing oxidative stress in a rat kidney model of ischemia-reperfusion injury. METHODS: Forty female rats underwent clamping of the left renal artery for 30 minutes, followed by reperfusion. The effects of pre-ischemic administration of NAC and/or ATOR were evaluated within 4 groups: a) control (no NAC, no ATOR); b) NAC (intraperitoneal NAC administration); c) ATOR (oral ATOR administration); and d) NAC+ATOR (both drugs). Oxidative stress was assessed by measuring the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and myeloperoxidase (MPO). Post-ischemia-reperfusion injury was evaluated by means of renal histology. RESULTS: NAC, ATOR, and NAC+ATOR in rats showed lower MPO (P < .05) and higher GPx activity (P < .05) versus control; SOD activity was lower in NAC versus ATOR (P < .05). No difference among groups was found at histology. However, a lower rate of tubular ischemic lesions was evident in NAC+ATOR versus control (P = .07). CONCLUSIONS: Atorvastatin pretreatment provides protection against oxidative stress in a rat kidney model of ischemia-reperfusion injury, reinforcing the evidence of a beneficial effect of statins beyond their cholesterol-lowering properties

Cola, E., Cusumano, G., Romagnoli, J., Copponi, G., Citterio, F., Caristo, M., Manchi, M., Severino, A., Liuzzo, G., Crea, F., Capelli, A., HIGH-DOSE ATORVASTATIN REDUCE OXIDATIVE STRESS OF ISCHEMIA/REPERFUSION INJURY AFTER ISOGENEIC KIDNEY TRANSPLANTATION IN RATS, <<TRANSPLANTATION PROCEEDINGS>>, 2015; 2015 (Novembre): 2757-2762. [doi:10.1016] [http://hdl.handle.net/10807/71796]

HIGH-DOSE ATORVASTATIN REDUCE OXIDATIVE STRESS OF ISCHEMIA/REPERFUSION INJURY AFTER ISOGENEIC KIDNEY TRANSPLANTATION IN RATS

Cusumano, Giacomo;Romagnoli, Jacopo;Copponi, Giorgia;Citterio, Franco;Manchi, Melissa;Severino, Anna;Liuzzo, Giovanna;Crea, Filippo;Capelli, Arnaldo
2015

Abstract

OBJECTIVE: To investigate the effects of N-acetylcysteine (NAC) and high-dose atorvastatin (ATOR) in reducing oxidative stress in a rat kidney model of ischemia-reperfusion injury. METHODS: Forty female rats underwent clamping of the left renal artery for 30 minutes, followed by reperfusion. The effects of pre-ischemic administration of NAC and/or ATOR were evaluated within 4 groups: a) control (no NAC, no ATOR); b) NAC (intraperitoneal NAC administration); c) ATOR (oral ATOR administration); and d) NAC+ATOR (both drugs). Oxidative stress was assessed by measuring the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and myeloperoxidase (MPO). Post-ischemia-reperfusion injury was evaluated by means of renal histology. RESULTS: NAC, ATOR, and NAC+ATOR in rats showed lower MPO (P < .05) and higher GPx activity (P < .05) versus control; SOD activity was lower in NAC versus ATOR (P < .05). No difference among groups was found at histology. However, a lower rate of tubular ischemic lesions was evident in NAC+ATOR versus control (P = .07). CONCLUSIONS: Atorvastatin pretreatment provides protection against oxidative stress in a rat kidney model of ischemia-reperfusion injury, reinforcing the evidence of a beneficial effect of statins beyond their cholesterol-lowering properties
Inglese
Cola, E., Cusumano, G., Romagnoli, J., Copponi, G., Citterio, F., Caristo, M., Manchi, M., Severino, A., Liuzzo, G., Crea, F., Capelli, A., HIGH-DOSE ATORVASTATIN REDUCE OXIDATIVE STRESS OF ISCHEMIA/REPERFUSION INJURY AFTER ISOGENEIC KIDNEY TRANSPLANTATION IN RATS, <<TRANSPLANTATION PROCEEDINGS>>, 2015; 2015 (Novembre): 2757-2762. [doi:10.1016] [http://hdl.handle.net/10807/71796]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10807/71796
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