The PTPN11 gene encodes SHP-2, a widely expressed cytoplasmic protein tyrosine phosphatase functioning as a signaling transducer. Germ-line PTPN11 mutations cause Noonan syndrome (NS), a developmental disorder characterized by an increased risk of malignancies. Recently, a novel class of activating mutations in PTPN11 has been documented as a somatic event in a heterogeneous group of leukemias. Because of the relatively higher prevalence of certain solid tumors in children with NS and the positive modulatory function of SHP-2 in RAS signaling, a wider role for activating PTPN11 mutations in cancer has been hypothesized. Here, we screened a number of solid tumors, including those documented in NS or in which deregulated RAS signaling occurs at significant frequency, for PTPN11 mutations. No disease-associated mutation was identified in rhabdomyosarcoma (n = 13), neuroblastoma (n = 32), melanoma (n = 50), thyroid (n = 85), and colon (n = 48) tumors; a novel missense change, promoting an increased basal phosphatase activity of SHP-2, was observed in one glioma specimen. Our data document that deregulated SHP-2 function does not represent a major molecular event in pediatric and adult tumors, further supporting our previous evidence indicating that the oncogenic role of PTPN11 mutations is cell-context specific.

Martinelli, S., Carta, C., Flex, E., Binni, F., Cordisco, E. L., Moretti, S., Puxeddu, E., Tonacchera, M., Pinchera, A., Mcdowell, H. P., Dominici, C., Rosolen, A., Di Rocco, C., Riccardi, R., Celli, P., Picardi, M., Genuardi, M., Grammatico, P., Sorcini, M., Tartaglia, M., Activating PTPN11 mutations play a minor role in pediatric and adult solid tumors, <<CANCER GENETICS AND CYTOGENETICS>>, 2006; 166 (2): 124-129. [doi:10.1016/j.cancergencyto.2005.10.003] [http://hdl.handle.net/10807/16301]

Activating PTPN11 mutations play a minor role in pediatric and adult solid tumors

Di Rocco, Concezio;Riccardi, Riccardo;Genuardi, Maurizio;Tartaglia, Marco
2006

Abstract

The PTPN11 gene encodes SHP-2, a widely expressed cytoplasmic protein tyrosine phosphatase functioning as a signaling transducer. Germ-line PTPN11 mutations cause Noonan syndrome (NS), a developmental disorder characterized by an increased risk of malignancies. Recently, a novel class of activating mutations in PTPN11 has been documented as a somatic event in a heterogeneous group of leukemias. Because of the relatively higher prevalence of certain solid tumors in children with NS and the positive modulatory function of SHP-2 in RAS signaling, a wider role for activating PTPN11 mutations in cancer has been hypothesized. Here, we screened a number of solid tumors, including those documented in NS or in which deregulated RAS signaling occurs at significant frequency, for PTPN11 mutations. No disease-associated mutation was identified in rhabdomyosarcoma (n = 13), neuroblastoma (n = 32), melanoma (n = 50), thyroid (n = 85), and colon (n = 48) tumors; a novel missense change, promoting an increased basal phosphatase activity of SHP-2, was observed in one glioma specimen. Our data document that deregulated SHP-2 function does not represent a major molecular event in pediatric and adult tumors, further supporting our previous evidence indicating that the oncogenic role of PTPN11 mutations is cell-context specific.
2006
Inglese
Martinelli, S., Carta, C., Flex, E., Binni, F., Cordisco, E. L., Moretti, S., Puxeddu, E., Tonacchera, M., Pinchera, A., Mcdowell, H. P., Dominici, C., Rosolen, A., Di Rocco, C., Riccardi, R., Celli, P., Picardi, M., Genuardi, M., Grammatico, P., Sorcini, M., Tartaglia, M., Activating PTPN11 mutations play a minor role in pediatric and adult solid tumors, <<CANCER GENETICS AND CYTOGENETICS>>, 2006; 166 (2): 124-129. [doi:10.1016/j.cancergencyto.2005.10.003] [http://hdl.handle.net/10807/16301]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/16301
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