A striking decrease of bovine kidney alkaline phosphatase activity is observed in vitro when the catalytic assay is performed after preincubation of the enzyme with ascorbic acid (AA). The inhibitory effect is a function of AA concentration time and on temperature. Activity decay follows an exponential biphasic course as a function of preincubation time composed by a "fast" phase in the first half hour and by a later "slow" phase of inhibition. Both the rise in preincubation temperature and the increase of the amount of vitamin enhance the degree of inhibition. Ascorbic acid is ineffective as inhibitor when added together with the substrate, p-nitrophenyl phosphate, which in fact markedly stabilizes the enzyme even when present in unsaturating amounts.

Miggiano, G., Mordente, A., Martorana, G., Meucci, E., Castelli, A., In vitro effect of ascorbic acid on bovine kidney alkaline phosphatase activity, <<ACTA VITAMINOLOGICA ET ENZYMOLOGICA>>, 1983; 5 (3): 153-158 [http://hdl.handle.net/10807/9766]

In vitro effect of ascorbic acid on bovine kidney alkaline phosphatase activity

Mordente, Alvaro;Meucci, Elisabetta;
1983

Abstract

A striking decrease of bovine kidney alkaline phosphatase activity is observed in vitro when the catalytic assay is performed after preincubation of the enzyme with ascorbic acid (AA). The inhibitory effect is a function of AA concentration time and on temperature. Activity decay follows an exponential biphasic course as a function of preincubation time composed by a "fast" phase in the first half hour and by a later "slow" phase of inhibition. Both the rise in preincubation temperature and the increase of the amount of vitamin enhance the degree of inhibition. Ascorbic acid is ineffective as inhibitor when added together with the substrate, p-nitrophenyl phosphate, which in fact markedly stabilizes the enzyme even when present in unsaturating amounts.
eng
Miggiano, G., Mordente, A., Martorana, G., Meucci, E., Castelli, A., In vitro effect of ascorbic acid on bovine kidney alkaline phosphatase activity, <>, 1983; 5 (3): 153-158 [http://hdl.handle.net/10807/9766]
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10807/9766
Citazioni
  • ???jsp.display-item.citation.pmc??? 2
  • Scopus 4
  • ???jsp.display-item.citation.isi??? ND
social impact