Ammonia oxidation has been intensively studied for its sensitivity to environmental shifts and stresses. However, acute stress effects on the occurrence and composition of ammonia oxidizing bacteria (AOB) and archaea (AOA) based on expression of related molecular markers in complex soil environments have been to an extent overlooked, particularly concerning transient but commonly occurring environmental changes like soil moisture shifts. The present study investigates the responses of AOB and AOA to moisture shifts and high Zn soil content. AmoA gene copies and transcripts of AOB and AOA along with potential nitrification activity were measured in a soil microcosm approach for investigating the referred environmental shifts. Moisture change from 87 to 50 % of the water holding capacity caused a ~99 % reduction of AOB but not of AOA amoA transcripts that did not change significantly. Increasing applied zinc concentrations resulted in a reduction of potential nitrification rates and negatively affected studied gene expressions of both AOB and AOA, with AOB being more responsive. Both 16 S rRNA and amoA transcripts of AOB had an inverse relation to the applied zinc, indicating a gradual loss in total cell activity. Our results suggest the existence of pronounced differences between AOB and AOA concerning ammonia oxidation activity.

Vasileiadis, S., Coppolecchia, D., Puglisi, E., Balloi, A., Mapelli, F., Hamon, R. E., Daffonchio, D., Trevisan, M., Response of Ammonia Oxidizing Bacteria and Archaea to Acute Zinc Stress and Different Moisture Regimes in Soil, <<MICROBIAL ECOLOGY>>, 2012; 64 (Novembre): 1028-1037. [doi:doi: 10.1007/s00248-012-0081-3] [http://hdl.handle.net/10807/41369]

Response of Ammonia Oxidizing Bacteria and Archaea to Acute Zinc Stress and Different Moisture Regimes in Soil

Vasileiadis, Sotirios;Coppolecchia, Damiano;Puglisi, Edoardo;Trevisan, Marco
2012

Abstract

Ammonia oxidation has been intensively studied for its sensitivity to environmental shifts and stresses. However, acute stress effects on the occurrence and composition of ammonia oxidizing bacteria (AOB) and archaea (AOA) based on expression of related molecular markers in complex soil environments have been to an extent overlooked, particularly concerning transient but commonly occurring environmental changes like soil moisture shifts. The present study investigates the responses of AOB and AOA to moisture shifts and high Zn soil content. AmoA gene copies and transcripts of AOB and AOA along with potential nitrification activity were measured in a soil microcosm approach for investigating the referred environmental shifts. Moisture change from 87 to 50 % of the water holding capacity caused a ~99 % reduction of AOB but not of AOA amoA transcripts that did not change significantly. Increasing applied zinc concentrations resulted in a reduction of potential nitrification rates and negatively affected studied gene expressions of both AOB and AOA, with AOB being more responsive. Both 16 S rRNA and amoA transcripts of AOB had an inverse relation to the applied zinc, indicating a gradual loss in total cell activity. Our results suggest the existence of pronounced differences between AOB and AOA concerning ammonia oxidation activity.
Inglese
Vasileiadis, S., Coppolecchia, D., Puglisi, E., Balloi, A., Mapelli, F., Hamon, R. E., Daffonchio, D., Trevisan, M., Response of Ammonia Oxidizing Bacteria and Archaea to Acute Zinc Stress and Different Moisture Regimes in Soil, <>, 2012; 64 (Novembre): 1028-1037. [doi:doi: 10.1007/s00248-012-0081-3] [http://hdl.handle.net/10807/41369]
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10807/41369
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 23
  • ???jsp.display-item.citation.isi??? 20
social impact