Salivary levels of alpha-defensins 1-4 and histatins 1, 3 and 5 were determined in 11 totally edentulous patients, 11 younger healthy adults with normal gingival mucosa (Control group 1) and 8 subjects, age-matched with edentulous patients, having a minimum of 25 teeth (Control group II). Whole saliva was treated with trifluoroacetic acid and the acidic soluble fraction analyzed by High Performance Liquid Chromatography-Mass Spectrometry. The area of the extracted ion current peaks was used for peptide quantification. Levels of alpha-defensinsl-4, but not of histatins, were significantly lower in totally edentulous patients with respect to both Control group I and Control group II. The two control groups did not show significant differences. The reduced level of oral alpha-defensins, which are mainly of crevicular origin, is most likely due to the absence of the gingival sulcus in the edentulous subjects. The near absence of alpha-defensins might be in part responsible for the higher vulnerability of the oral cavity to oral pathogen infections observed in totally edentulous patients.

Fanali, C., Inzitari, R., Cabras, T., Pisano, E., Castagnola, M., Celletti, R., Manni, A., Messana, I., Alfa-Defensins levels in whole saliva of totally edentulous subjects, <<INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY>>, 2008; (21): 845-849 [http://hdl.handle.net/10807/25637]

Alfa-Defensins levels in whole saliva of totally edentulous subjects

Fanali, Chiara;Inzitari, Rosanna;Cabras, Tiziana;Pisano, Elisabetta;Castagnola, Massimo;Manni, Armando;Messana, Irene
2008

Abstract

Salivary levels of alpha-defensins 1-4 and histatins 1, 3 and 5 were determined in 11 totally edentulous patients, 11 younger healthy adults with normal gingival mucosa (Control group 1) and 8 subjects, age-matched with edentulous patients, having a minimum of 25 teeth (Control group II). Whole saliva was treated with trifluoroacetic acid and the acidic soluble fraction analyzed by High Performance Liquid Chromatography-Mass Spectrometry. The area of the extracted ion current peaks was used for peptide quantification. Levels of alpha-defensinsl-4, but not of histatins, were significantly lower in totally edentulous patients with respect to both Control group I and Control group II. The two control groups did not show significant differences. The reduced level of oral alpha-defensins, which are mainly of crevicular origin, is most likely due to the absence of the gingival sulcus in the edentulous subjects. The near absence of alpha-defensins might be in part responsible for the higher vulnerability of the oral cavity to oral pathogen infections observed in totally edentulous patients.
Inglese
Fanali, C., Inzitari, R., Cabras, T., Pisano, E., Castagnola, M., Celletti, R., Manni, A., Messana, I., Alfa-Defensins levels in whole saliva of totally edentulous subjects, <<INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY>>, 2008; (21): 845-849 [http://hdl.handle.net/10807/25637]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/25637
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