Thirteen samples of human normal whole saliva were analyzed by RP-HPLC-ESI-MS and MALDI-TOF-MS to investigate the basic proline-rich protein complex. Between known basic-PRPs the P-B, P-C (or IB-8b), P-D (or IB-5), P-E (or IB-9), P-F (or IB-8), P-H (or IB-4), IB-6,II-2, IB-1, and IB-8a glucosylated were identified, whereas the II-1, IB-7, PA, and D1-A peptides were not detected. Some detected masses not attributable to known basic-PRPs were putatively ascribed to II-2 and IB-1 nonphosphorylated, II-2 and IB-1 missing the C-terminal arginine residue, and the 1-62 fragment of IB-6, named P-J peptide. A correlation matrix analysis revealed a cluster of correlation among all the basic PRF's (apart from the P-B peptide) which is in agreement with their common parotid origin.

Messana, I., Cabras, T., Inzitari, R., Lupi, A., Zuppi, C., Olmi, C., Fadda, M. B., Cordaro, M., Giardina, B., Castagnola, M., Characterization of the human salivary basic proline-rich protein complex by a proteomic approach, <<JOURNAL OF PROTEOME RESEARCH>>, 2004; (3): 792-800 [http://hdl.handle.net/10807/25605]

Characterization of the human salivary basic proline-rich protein complex by a proteomic approach

Messana, Irene;Cabras, Tiziana;Inzitari, Rosanna;Lupi, Alessandro;Zuppi, Cecilia;Olmi, Chiara;Fadda, Maria Benedetta;Cordaro, Massimo;Giardina, Bruno;Castagnola, Massimo
2004

Abstract

Thirteen samples of human normal whole saliva were analyzed by RP-HPLC-ESI-MS and MALDI-TOF-MS to investigate the basic proline-rich protein complex. Between known basic-PRPs the P-B, P-C (or IB-8b), P-D (or IB-5), P-E (or IB-9), P-F (or IB-8), P-H (or IB-4), IB-6,II-2, IB-1, and IB-8a glucosylated were identified, whereas the II-1, IB-7, PA, and D1-A peptides were not detected. Some detected masses not attributable to known basic-PRPs were putatively ascribed to II-2 and IB-1 nonphosphorylated, II-2 and IB-1 missing the C-terminal arginine residue, and the 1-62 fragment of IB-6, named P-J peptide. A correlation matrix analysis revealed a cluster of correlation among all the basic PRF's (apart from the P-B peptide) which is in agreement with their common parotid origin.
Inglese
Messana, I., Cabras, T., Inzitari, R., Lupi, A., Zuppi, C., Olmi, C., Fadda, M. B., Cordaro, M., Giardina, B., Castagnola, M., Characterization of the human salivary basic proline-rich protein complex by a proteomic approach, <<JOURNAL OF PROTEOME RESEARCH>>, 2004; (3): 792-800 [http://hdl.handle.net/10807/25605]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/25605
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