Objective: to assess the influence of SARS-CoV-2 mRNA vaccine on B-cell phenotypes in systemic sclerosis (SSc). Methods: peripheral blood B-cell subpopulations were evaluated before (t1) and 3 months (t3) after the second dose of vaccine in 28 SSc patients. Peripheral blood B-cell subpopulations were evaluated in 21 healthy controls (HCs) only at t1. Anti-spike IgG levels were evaluated at t3 in both cohorts. Results: SSc patients presented higher naive, double-negative, and CD21(low) B cells compared to HCs. IgM-memory and switched-memory B cells were lower in SSc patients than HCs. No differences in anti-spike IgG levels after vaccination were observed between SSc patients and HCs. Anti-spike IgG levels after vaccination were lower in SSc patients with increased CD21(low) B cells at baseline compared to SSc patients with normal CD21(low) B cells. A positive correlation was found between IgG levels and naive B cells. A negative linear correlation was shown between IgG levels and IgM-memory, switched-memory, double-negative, and CD21(low) B cells. Conclusions: SARS-CoV-2 mRNA vaccine response is normal in SSc patients not undergoing immunosuppressive therapy. The normal number of naive B cells is a positive marker of antibody response. The increased percentage of CD21(low) B cells represents a negative marker of antibody response.

Pellicano, C., Colalillo, A., Basile, V., Marino, M., Basile, U., La Gualana, F., Mezzaroma, I., Visentini, M., Rosato, E., The Effect of SARS-CoV-2 Vaccination on B-Cell Phenotype in Systemic Sclerosis Patients, <<JOURNAL OF PERSONALIZED MEDICINE>>, 2022; 12 (9): 1420-1430. [doi:10.3390/jpm12091420] [https://hdl.handle.net/10807/222007]

The Effect of SARS-CoV-2 Vaccination on B-Cell Phenotype in Systemic Sclerosis Patients

Marino, Mariapaola;Basile, Umberto
;
2022

Abstract

Objective: to assess the influence of SARS-CoV-2 mRNA vaccine on B-cell phenotypes in systemic sclerosis (SSc). Methods: peripheral blood B-cell subpopulations were evaluated before (t1) and 3 months (t3) after the second dose of vaccine in 28 SSc patients. Peripheral blood B-cell subpopulations were evaluated in 21 healthy controls (HCs) only at t1. Anti-spike IgG levels were evaluated at t3 in both cohorts. Results: SSc patients presented higher naive, double-negative, and CD21(low) B cells compared to HCs. IgM-memory and switched-memory B cells were lower in SSc patients than HCs. No differences in anti-spike IgG levels after vaccination were observed between SSc patients and HCs. Anti-spike IgG levels after vaccination were lower in SSc patients with increased CD21(low) B cells at baseline compared to SSc patients with normal CD21(low) B cells. A positive correlation was found between IgG levels and naive B cells. A negative linear correlation was shown between IgG levels and IgM-memory, switched-memory, double-negative, and CD21(low) B cells. Conclusions: SARS-CoV-2 mRNA vaccine response is normal in SSc patients not undergoing immunosuppressive therapy. The normal number of naive B cells is a positive marker of antibody response. The increased percentage of CD21(low) B cells represents a negative marker of antibody response.
Inglese
Pellicano, C., Colalillo, A., Basile, V., Marino, M., Basile, U., La Gualana, F., Mezzaroma, I., Visentini, M., Rosato, E., The Effect of SARS-CoV-2 Vaccination on B-Cell Phenotype in Systemic Sclerosis Patients, <<JOURNAL OF PERSONALIZED MEDICINE>>, 2022; 12 (9): 1420-1430. [doi:10.3390/jpm12091420] [https://hdl.handle.net/10807/222007]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/222007
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