Olive (Olea europaea L.) is one of the most important fruit species in the Mediterranean basin, where 95% of the world's olive orchards are planted, and has become an economically valuable crop worldwide due to an increasing demand in olive oil for human consumption. The aim of the present research was to classify Tunisian olive cultivars based on their phenolic profile and to test the potentiality of chemometrics as a tool for cultivars discrimination. The phenolic compounds present in 21 samples of olive leaves, collected during the normal picking period for olive oil production in South Tunisia, were analysed by liquid chromatography mass spectrometry. Models based on principal component analysis (PCA) and hierarchical cluster analysis (HCA) was used for discrimination purposes as a function of the tree varieties and cultivation zone. Qualitative and quantitative compositional analysis revealed 12 major phenolic compounds among which oleuropein (110.38–4741.62 mg/kg), hydroxytyrosol (5.40–290.19 mg/kg), apigenin 7-O-glucoside (9.44–2475.53 mg/kg), tyrosol (2.10–174.62 mg/kg), catechin (0–37.60 mg/kg) and vanillic acid (3.23–256.57 mg/kg) were identified. Oleuropein showed the highest amount in Fougi cultivar (4741.62 mg/kg), whereas the lowest level was registered in Zarrazi injassi cultivar (110.38 mg/kg). However, Zalmati Zarzis exhibited high concentrations of tyrosol, vanillic and caffeic acid, and vanillin. In general, rutin and luteolin 7-Oglucoside were the two main flavonoids detected. Dendrogram analysis revealed three main clusters, further confirmed through PCA.

Ben Mohamed, M., Guasmi, F., Ben Ali, S., Radhouani, F., Faghim, J., Triki, T., Kammoun, N. G., Baffi, C., Lucini, L., Benincasa, C., The LC-MS/MS characterization of phenolic compounds in leaves allows classifying olive cultivars grown in South Tunisia, <<BIOCHEMICAL SYSTEMATICS AND ECOLOGY>>, 2018; 78 (78): 84-90. [doi:10.1016/j.bse.2018.04.005] [http://hdl.handle.net/10807/122971]

The LC-MS/MS characterization of phenolic compounds in leaves allows classifying olive cultivars grown in South Tunisia

Baffi, Claudio;Lucini, Luigi;
2018

Abstract

Olive (Olea europaea L.) is one of the most important fruit species in the Mediterranean basin, where 95% of the world's olive orchards are planted, and has become an economically valuable crop worldwide due to an increasing demand in olive oil for human consumption. The aim of the present research was to classify Tunisian olive cultivars based on their phenolic profile and to test the potentiality of chemometrics as a tool for cultivars discrimination. The phenolic compounds present in 21 samples of olive leaves, collected during the normal picking period for olive oil production in South Tunisia, were analysed by liquid chromatography mass spectrometry. Models based on principal component analysis (PCA) and hierarchical cluster analysis (HCA) was used for discrimination purposes as a function of the tree varieties and cultivation zone. Qualitative and quantitative compositional analysis revealed 12 major phenolic compounds among which oleuropein (110.38–4741.62 mg/kg), hydroxytyrosol (5.40–290.19 mg/kg), apigenin 7-O-glucoside (9.44–2475.53 mg/kg), tyrosol (2.10–174.62 mg/kg), catechin (0–37.60 mg/kg) and vanillic acid (3.23–256.57 mg/kg) were identified. Oleuropein showed the highest amount in Fougi cultivar (4741.62 mg/kg), whereas the lowest level was registered in Zarrazi injassi cultivar (110.38 mg/kg). However, Zalmati Zarzis exhibited high concentrations of tyrosol, vanillic and caffeic acid, and vanillin. In general, rutin and luteolin 7-Oglucoside were the two main flavonoids detected. Dendrogram analysis revealed three main clusters, further confirmed through PCA.
Inglese
Ben Mohamed, M., Guasmi, F., Ben Ali, S., Radhouani, F., Faghim, J., Triki, T., Kammoun, N. G., Baffi, C., Lucini, L., Benincasa, C., The LC-MS/MS characterization of phenolic compounds in leaves allows classifying olive cultivars grown in South Tunisia, <<BIOCHEMICAL SYSTEMATICS AND ECOLOGY>>, 2018; 78 (78): 84-90. [doi:10.1016/j.bse.2018.04.005] [http://hdl.handle.net/10807/122971]
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/122971
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 33
  • ???jsp.display-item.citation.isi??? 31
social impact