A salivary proline-rich peptide of 1932 Da showed a dose-dependent antagonistic effect on the cytosolic Ca2+ mobilization induced by progesterone in a tongue squamous carcinoma cell line. Structure-activity studies showed that the activity of the peptide resides in the C-terminal region characterized by a proline stretch flanked by basic residues. Furthermore, lack of activity of the retro-inverso peptide analogue suggested the involvement of stereospecific recognition. Mass spectrometry-based shotgun analysis, combined with Western blotting tests and biochemical data obtained with the Progesterone Receptor Membrane Component 1 (PGRMC1) inhibitor AG205, showed strong evidence that p1932 performs its modulatory action through an interaction with the progesterone receptor PGRMC1, which is predominantly expressed in this cell line and, clearly, plays a role in progesterone induced Ca2+ response. Thus, our results point to p1932 as a modulator of the transduction signal pathway mediated by this protein and, given a well-established involvement of PGRMC1 in tumorigenesis, highlight a possible therapeutic potential of p1932 for the treatment of oral cancer.

Palmerini, C. A., Mazzoni, M., Radicioni, G., Marzano, V., Granieri, L., Iavarone, F., Longhi, R., Messana, I., Cabras, T., Sanna, M. T., Castagnola, M., Vitali, A., Antagonistic Effect of a Salivary Proline-Rich Peptide on the Cytosolic Ca2+ Mobilization Induced by Progesterone in Oral Squamous Cancer Cells., <<PLOS ONE>>, 2016; 2016 (11(1)): e0147925.-e0147925. [http://hdl.handle.net/10807/96012]

Antagonistic Effect of a Salivary Proline-Rich Peptide on the Cytosolic Ca2+ Mobilization Induced by Progesterone in Oral Squamous Cancer Cells.

Radicioni, Giorgia;Marzano, Valeria;Granieri, Letizia;Iavarone, Federica;Messana, Irene;Cabras, Tiziana;Sanna, Maria Teresa;Castagnola, Massimo
Penultimo
;
Vitali, Alberto
Ultimo
2016

Abstract

A salivary proline-rich peptide of 1932 Da showed a dose-dependent antagonistic effect on the cytosolic Ca2+ mobilization induced by progesterone in a tongue squamous carcinoma cell line. Structure-activity studies showed that the activity of the peptide resides in the C-terminal region characterized by a proline stretch flanked by basic residues. Furthermore, lack of activity of the retro-inverso peptide analogue suggested the involvement of stereospecific recognition. Mass spectrometry-based shotgun analysis, combined with Western blotting tests and biochemical data obtained with the Progesterone Receptor Membrane Component 1 (PGRMC1) inhibitor AG205, showed strong evidence that p1932 performs its modulatory action through an interaction with the progesterone receptor PGRMC1, which is predominantly expressed in this cell line and, clearly, plays a role in progesterone induced Ca2+ response. Thus, our results point to p1932 as a modulator of the transduction signal pathway mediated by this protein and, given a well-established involvement of PGRMC1 in tumorigenesis, highlight a possible therapeutic potential of p1932 for the treatment of oral cancer.
Inglese
Palmerini, C. A., Mazzoni, M., Radicioni, G., Marzano, V., Granieri, L., Iavarone, F., Longhi, R., Messana, I., Cabras, T., Sanna, M. T., Castagnola, M., Vitali, A., Antagonistic Effect of a Salivary Proline-Rich Peptide on the Cytosolic Ca2+ Mobilization Induced by Progesterone in Oral Squamous Cancer Cells., <<PLOS ONE>>, 2016; 2016 (11(1)): e0147925.-e0147925. [http://hdl.handle.net/10807/96012]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10807/96012
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