The paranasal sinus epithelium is exposed to the environment and therefore to a variety of biological, chemical and mechanical insults. Surfactant protein A (SP-A) is a 34–36 kD pulmonary surfactant-associated protein that appears to play an important role in mammalian first-line host defence. Recent studies have reported the possibility of local production of SP-A in the extrapulmonary organs and tissues of the human body. However, the presence of SP-A in the human paranasal sinus mucosa is not well known. The purpose of this study was to investigate the expression of SP-A protein in human turbinate mucosa and to compare the expression of SP-A mRNA in normal turbinate mucosa and turbinate mucosa of chronic rhinosinusitis patients. Reverse transcriptase polymerase chain reaction was used to detect SP-A mRNA. Student’s t test was used for statistical comparison of the SP-A/GAPDH-mRNA ratio (GAPDH: glycerinaldehyde-3-phosphate dehydrogenase) of cases and controls. We found expression of SP-A mRNA in mucosa lining the inferior turbinates of healthy patients and its up-regulation in mucosa lining the inferior turbinates of patients with chronic rhinosinusitis. These results may provide targets for new therapies for chronic rhinosinusitis.

Passali, D., Astore, S., Boccuzzi, S., Loglisci, M., Cannatelli, A., Passali, G. C., Bellussi, L., Werte für SP-A-Protein in der nasalen Mukosa, <<HNO>>, 2016; 64 (9): 671-675. [doi:10.1007/s00106-016-0190-2] [http://hdl.handle.net/10807/95629]

Werte für SP-A-Protein in der nasalen Mukosa

Passali, Giulio Cesare
Penultimo
;
2016

Abstract

The paranasal sinus epithelium is exposed to the environment and therefore to a variety of biological, chemical and mechanical insults. Surfactant protein A (SP-A) is a 34–36 kD pulmonary surfactant-associated protein that appears to play an important role in mammalian first-line host defence. Recent studies have reported the possibility of local production of SP-A in the extrapulmonary organs and tissues of the human body. However, the presence of SP-A in the human paranasal sinus mucosa is not well known. The purpose of this study was to investigate the expression of SP-A protein in human turbinate mucosa and to compare the expression of SP-A mRNA in normal turbinate mucosa and turbinate mucosa of chronic rhinosinusitis patients. Reverse transcriptase polymerase chain reaction was used to detect SP-A mRNA. Student’s t test was used for statistical comparison of the SP-A/GAPDH-mRNA ratio (GAPDH: glycerinaldehyde-3-phosphate dehydrogenase) of cases and controls. We found expression of SP-A mRNA in mucosa lining the inferior turbinates of healthy patients and its up-regulation in mucosa lining the inferior turbinates of patients with chronic rhinosinusitis. These results may provide targets for new therapies for chronic rhinosinusitis.
Inglese
HNO
link.springer.de/link/service/journals/00106/index.htm
Passali, D., Astore, S., Boccuzzi, S., Loglisci, M., Cannatelli, A., Passali, G. C., Bellussi, L., Werte für SP-A-Protein in der nasalen Mukosa, <>, 2016; 64 (9): 671-675. [doi:10.1007/s00106-016-0190-2] [http://hdl.handle.net/10807/95629]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10807/95629
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