BACKGROUND: The immaturity of immune system characterizes newborn infants. Possible serological markers of Th1 and Th2 immune response are the lymphocyte activation gene-3 (CD223) and soluble CD30, respectively (sCD30). AIMS: The aim of our study was to evaluate the relationship between Th1 and Th2 immune response and gestational age (GA), comparing data in preterm and term neonates. STUDY DESIGN: Cord blood from 20 preterm (GA: 33±2weeks, BW 1950±490g) and 20 term infants (GA: 38±1weeks, BW: 3177±330g) were tested for sCD30 and CD223 levels by ELISA. IFNγ levels produced by cord blood lymphocytes were also analyzed, both before and after stimulation with phytohaemagglutinin (PHA). RESULTS: sCD30 resulted significantly higher in preterm neonates when compared with term neonates (60±7.6 vs 42.6±3.9U/ml p<0.05). CD223 was undetectable in preterm neonates while resulting at a level of 176.1±112.6ng/ml in term neonates. After stimulation with PHA, a significant increase in IFNγ levels was only observed in term neonates (326.6±72.7pg/ml p<0.05). CONCLUSIONS: Our findings show that sCD30 is present and measurable in term and preterm infants, while CD223 is detectable only in term infants and that Th-cell polarization could also depend on gestational age. Our data suggest that a Th2 immune response seems predominant in preterm neonates.

Frezza, S., Gallini, F., Palazzo, R., Carollo, M., De Carolis, M. P., D'andrea, V., Catenazzi, P., Romagnoli, C., Ausiello, C. M., T-cell polarization: Potential serological markers in preterm and term infants., <<EARLY HUMAN DEVELOPMENT>>, 2016; (Oct;101): 69-71. [doi:10.1016/j.earlhumdev.2016.03.013] [http://hdl.handle.net/10807/95192]

T-cell polarization: Potential serological markers in preterm and term infants.

Frezza, Simonetta
Primo
;
Gallini, Francesca
Secondo
;
De Carolis, Maria Pia;D'Andrea, Vito;Catenazzi, Piero;Romagnoli, Costantino
Penultimo
;
2016

Abstract

BACKGROUND: The immaturity of immune system characterizes newborn infants. Possible serological markers of Th1 and Th2 immune response are the lymphocyte activation gene-3 (CD223) and soluble CD30, respectively (sCD30). AIMS: The aim of our study was to evaluate the relationship between Th1 and Th2 immune response and gestational age (GA), comparing data in preterm and term neonates. STUDY DESIGN: Cord blood from 20 preterm (GA: 33±2weeks, BW 1950±490g) and 20 term infants (GA: 38±1weeks, BW: 3177±330g) were tested for sCD30 and CD223 levels by ELISA. IFNγ levels produced by cord blood lymphocytes were also analyzed, both before and after stimulation with phytohaemagglutinin (PHA). RESULTS: sCD30 resulted significantly higher in preterm neonates when compared with term neonates (60±7.6 vs 42.6±3.9U/ml p<0.05). CD223 was undetectable in preterm neonates while resulting at a level of 176.1±112.6ng/ml in term neonates. After stimulation with PHA, a significant increase in IFNγ levels was only observed in term neonates (326.6±72.7pg/ml p<0.05). CONCLUSIONS: Our findings show that sCD30 is present and measurable in term and preterm infants, while CD223 is detectable only in term infants and that Th-cell polarization could also depend on gestational age. Our data suggest that a Th2 immune response seems predominant in preterm neonates.
Inglese
Frezza, S., Gallini, F., Palazzo, R., Carollo, M., De Carolis, M. P., D'andrea, V., Catenazzi, P., Romagnoli, C., Ausiello, C. M., T-cell polarization: Potential serological markers in preterm and term infants., <<EARLY HUMAN DEVELOPMENT>>, 2016; (Oct;101): 69-71. [doi:10.1016/j.earlhumdev.2016.03.013] [http://hdl.handle.net/10807/95192]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10807/95192
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