There is a real medical need of new diagnostic tools for the early recognition of invasive Candida infections. We exploited a rather simple and rapid redox methodology to construct a bispecific monoclonal antibody (bsmAb) that combines a monoclonal antibody (mAb) directed against 1,3-β-D-glucan, a well-known, pan-fungal diagnostic biomarker, with a mAb recognizing MP65, a major immunogenic mannoprotein secreted by C.albicans and other Candida species. The bsmAb (MP65/bglu mAb) was successfully produced and purified at high yields and proved to bind and reveal simultaneously, with high sensitivity, the β- glucan and MP65 antigens in both purified and native forms. The MP65/bglu mAb is the first bispecific antibody generated against a fungal microorganism and may prove useful for the concurrent detection of different and clinically significant Candida biomarkers in patient sera.

Zito, A., Bromuro, C., Mandili, G., Chiani, P., Horenstein, A. L., Malavasi, F., Cauda, R., Cassone, A., Torosantucci, A., A murine, bispecific monoclonal antibody simultaneously recognizing β-Glucan and MP65 determinants in Candida species, <<PLOS ONE>>, 2016; 11 (2): e0148714-N/A. [doi:10.1371/journal.pone.0148714] [http://hdl.handle.net/10807/95086]

A murine, bispecific monoclonal antibody simultaneously recognizing β-Glucan and MP65 determinants in Candida species

Cauda, Roberto;
2016

Abstract

There is a real medical need of new diagnostic tools for the early recognition of invasive Candida infections. We exploited a rather simple and rapid redox methodology to construct a bispecific monoclonal antibody (bsmAb) that combines a monoclonal antibody (mAb) directed against 1,3-β-D-glucan, a well-known, pan-fungal diagnostic biomarker, with a mAb recognizing MP65, a major immunogenic mannoprotein secreted by C.albicans and other Candida species. The bsmAb (MP65/bglu mAb) was successfully produced and purified at high yields and proved to bind and reveal simultaneously, with high sensitivity, the β- glucan and MP65 antigens in both purified and native forms. The MP65/bglu mAb is the first bispecific antibody generated against a fungal microorganism and may prove useful for the concurrent detection of different and clinically significant Candida biomarkers in patient sera.
Inglese
http://www.plosone.org/article/fetchObject.action?uri=info:doi/10.1371/journal.pone.0148714&representation=PDF
Zito, A., Bromuro, C., Mandili, G., Chiani, P., Horenstein, A. L., Malavasi, F., Cauda, R., Cassone, A., Torosantucci, A., A murine, bispecific monoclonal antibody simultaneously recognizing β-Glucan and MP65 determinants in Candida species, <>, 2016; 11 (2): e0148714-N/A. [doi:10.1371/journal.pone.0148714] [http://hdl.handle.net/10807/95086]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10807/95086
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