Metabolic surgery improves insulin resistance and type 2 diabetes possibly because of weight loss. We performed a novel sleeve gastrectomy in rats that resects ∼80% of the glandular portion, leaving the forestomach almost intact (glandular gastrectomy [GG]) and compared subsequent metabolic remodeling with a sham operation. GG did not affect body weight, at least after 10 weeks; improved hepatic and peripheral insulin sensitivity likely through increased Akt, glycogen synthase kinase 3, and AMPK phosphorylation; and reduced ectopic fat deposition and hepatic glycogen overaccumulation. Body adipose tissue was redistributed, with reduction of intraabdominal fat. We found a reduction of circulating ghrelin levels, increased GLP-1 plasma concentration, and remodeling of gut microbiome diversity characterized by a lower relative abundance of Ruminococcus and a higher relative abundance of Lactobacillus and Collinsella. These data suggest that at least in rat, the glandular stomach plays a central role in the improvement of insulin resistance, even if obesity persists. GG provides a new model of the metabolically healthy obese phenotype.

Basso, N., Soricelli, E., Castagneto Gissey, L., Casella, G., Albanese, D., Fava, F., Donati, C., Tuohy, K., Angelini, G., Neve, F. L., Severino, A., Kamvissi Lorenz, V., Birkenfeld, A. L., Bornstein, S., Manco, M., Mingrone, G., Insulin resistance, microbiota, and fat distribution changes by a new model of vertical sleeve gastrectomy in obese rats, <<DIABETES>>, 2016; 65 (10): 2990-3001. [doi:10.2337/db16-0039] [http://hdl.handle.net/10807/93115]

Insulin resistance, microbiota, and fat distribution changes by a new model of vertical sleeve gastrectomy in obese rats

Angelini, Giulia;Severino, Anna;Manco, Melania
Penultimo
;
Mingrone, Geltrude
Ultimo
2016

Abstract

Metabolic surgery improves insulin resistance and type 2 diabetes possibly because of weight loss. We performed a novel sleeve gastrectomy in rats that resects ∼80% of the glandular portion, leaving the forestomach almost intact (glandular gastrectomy [GG]) and compared subsequent metabolic remodeling with a sham operation. GG did not affect body weight, at least after 10 weeks; improved hepatic and peripheral insulin sensitivity likely through increased Akt, glycogen synthase kinase 3, and AMPK phosphorylation; and reduced ectopic fat deposition and hepatic glycogen overaccumulation. Body adipose tissue was redistributed, with reduction of intraabdominal fat. We found a reduction of circulating ghrelin levels, increased GLP-1 plasma concentration, and remodeling of gut microbiome diversity characterized by a lower relative abundance of Ruminococcus and a higher relative abundance of Lactobacillus and Collinsella. These data suggest that at least in rat, the glandular stomach plays a central role in the improvement of insulin resistance, even if obesity persists. GG provides a new model of the metabolically healthy obese phenotype.
Inglese
Basso, N., Soricelli, E., Castagneto Gissey, L., Casella, G., Albanese, D., Fava, F., Donati, C., Tuohy, K., Angelini, G., Neve, F. L., Severino, A., Kamvissi Lorenz, V., Birkenfeld, A. L., Bornstein, S., Manco, M., Mingrone, G., Insulin resistance, microbiota, and fat distribution changes by a new model of vertical sleeve gastrectomy in obese rats, <<DIABETES>>, 2016; 65 (10): 2990-3001. [doi:10.2337/db16-0039] [http://hdl.handle.net/10807/93115]
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/93115
Citazioni
  • ???jsp.display-item.citation.pmc??? 18
  • Scopus 37
  • ???jsp.display-item.citation.isi??? 30
social impact