In bone tissue reconstruction, the use of engineered constructs created by mesenchymal stem cells (MSCs) that differentiate and proliferate into 3D porous scaffolds is an appealing alternative to clinical therapies. Human placenta represents a possible source of MSCs, as it is readily available without invasive procedures and because of the phenotypic plasticity of many of the cell types isolated from this tissue. The scaffold considered in this work is a slowly degradable polyurethane foam (EF PU foam), synthesized and characterized for morphology and in vitro interaction with chorion mesenchymal cells (CMCs). These cells were isolated from human term placenta and cultured onto the EF PU foam using two different culture media (EMEM and NH osteogenic differentiation medium). Synthesized EF PU foam showed homogeneous pore size and distribution, with 89% open porosity. In vitro tests showed CMCs scaffold colonization, as confirmed by Scanning Electron Microscopy (SEM) observations and hematoxylin-eosin staining. Alizarin Red staining revealed the presence of a small amount of calcium deposition for the samples treated with the osteogenic differentiation medium. Therefore, the proposed EF PU foam appears to stimulate cell adhesion in vitro, sustaining CMCs growth and differentiation into the osteogenic lineage.

Bertoldi, S., Farè, S., Denegri, M., Rossi, D., Haugen, H. J., Parolini, O., Tanzi, M. C., Ability of polyurethane foams to support placenta-derived cell adhesion and osteogenic differentiation: preliminary results, <<JOURNAL OF MATERIALS SCIENCE. MATERIALS IN MEDICINE>>, 2010; 21 (3): 1005-1011. [doi:10.1007/s10856-009-3953-4] [http://hdl.handle.net/10807/92444]

Ability of polyurethane foams to support placenta-derived cell adhesion and osteogenic differentiation: preliminary results

Rossi, Daniele;Parolini, Ornella
Penultimo
;
2010

Abstract

In bone tissue reconstruction, the use of engineered constructs created by mesenchymal stem cells (MSCs) that differentiate and proliferate into 3D porous scaffolds is an appealing alternative to clinical therapies. Human placenta represents a possible source of MSCs, as it is readily available without invasive procedures and because of the phenotypic plasticity of many of the cell types isolated from this tissue. The scaffold considered in this work is a slowly degradable polyurethane foam (EF PU foam), synthesized and characterized for morphology and in vitro interaction with chorion mesenchymal cells (CMCs). These cells were isolated from human term placenta and cultured onto the EF PU foam using two different culture media (EMEM and NH osteogenic differentiation medium). Synthesized EF PU foam showed homogeneous pore size and distribution, with 89% open porosity. In vitro tests showed CMCs scaffold colonization, as confirmed by Scanning Electron Microscopy (SEM) observations and hematoxylin-eosin staining. Alizarin Red staining revealed the presence of a small amount of calcium deposition for the samples treated with the osteogenic differentiation medium. Therefore, the proposed EF PU foam appears to stimulate cell adhesion in vitro, sustaining CMCs growth and differentiation into the osteogenic lineage.
Inglese
Bertoldi, S., Farè, S., Denegri, M., Rossi, D., Haugen, H. J., Parolini, O., Tanzi, M. C., Ability of polyurethane foams to support placenta-derived cell adhesion and osteogenic differentiation: preliminary results, <>, 2010; 21 (3): 1005-1011. [doi:10.1007/s10856-009-3953-4] [http://hdl.handle.net/10807/92444]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10807/92444
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