Circulating Endothelial Progenitor Cells (EPCs) were discovered by Asahara et al in 1997 and defined as bone marrow CD34+/KDR+ cells endowed with angiogenic potentialities in vitro and in vivo. The most likely assumption is that EPCs consist of several cell subpopulations with functions targeted at accomplishing the post-natal neovascularization process in a synergic and complementary fashion. Indeed, the subsequent identification of numerous and differentiated hematic populations, characterized by the capacity to develop an endothelial phenotype, has posed a number of questions as to the real identity of EPCs. This concept does not represent a sterile speculation but rather it suggests important implications for the future practice of stem cell therapy. The aim of this report was to explore through a critical analysis the two main experimental methodologies, in vitro culture and flow cytometry, applied to EPCs, followed by a brief revaluation of the endothelial progenitors employing a globally functional approach.

Zaccone, V., Flore, R. A., Santoro, L., De Matteis, G., Giupponi, B., Li Puma, D. D., Santoliquido, A., Focus on biological identity of endothelial progenitors cells, <<EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES>>, 2015; 19 (21): 4047-4063 [http://hdl.handle.net/10807/71571]

Focus on biological identity of endothelial progenitors cells

Zaccone;Vincenzo; Flore;Roberto Antonio; Santoro;Luca; De Matteis;Giuseppe; Giupponi;Bianca; Li Puma;Domenica Donatella; Santoliquido
2015

Abstract

Circulating Endothelial Progenitor Cells (EPCs) were discovered by Asahara et al in 1997 and defined as bone marrow CD34+/KDR+ cells endowed with angiogenic potentialities in vitro and in vivo. The most likely assumption is that EPCs consist of several cell subpopulations with functions targeted at accomplishing the post-natal neovascularization process in a synergic and complementary fashion. Indeed, the subsequent identification of numerous and differentiated hematic populations, characterized by the capacity to develop an endothelial phenotype, has posed a number of questions as to the real identity of EPCs. This concept does not represent a sterile speculation but rather it suggests important implications for the future practice of stem cell therapy. The aim of this report was to explore through a critical analysis the two main experimental methodologies, in vitro culture and flow cytometry, applied to EPCs, followed by a brief revaluation of the endothelial progenitors employing a globally functional approach.
Inglese
Zaccone, V., Flore, R. A., Santoro, L., De Matteis, G., Giupponi, B., Li Puma, D. D., Santoliquido, A., Focus on biological identity of endothelial progenitors cells, <<EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES>>, 2015; 19 (21): 4047-4063 [http://hdl.handle.net/10807/71571]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/71571
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