Rheumatoid arthritis (RA) is characterized by chronic joint inflammation and associates with HLA-DRB1*04. The Collagen IIp261-273-specific T cell repertoire in the peripheral blood of DR4+ patients at the onset of the disease shows a restricted TCR-beta chain usage among which the most frequent is TRBV25. To define whether this group of DR4-restricted collagen-specific shared T cell could represent markers of activesevere disease and response to therapy, 90 subjects affected by early-RA were enrolled in the study; peripheral blood mononuclear cells were cultured with or without the human collagen II peptide p261-273 and were examined by immunoscope analysis for the usage of the previously identified shared TCR-beta chains. We report that the presence of T cells carrying rearrangement TRBV25 associated with HLA-DR haplotype and disease activity. HLA-DRB1* haplotypes 04–04, 04–01 and 04–11 were significantly associated with usage of TRBV25, higher disease activity at the onset of disease and poor response to DMARDs. Finally, the HLA-DRB1* haplotype appeared complementary with current serologic tools to predict good and poor responders in a treat to target strategy. The data reported here offer clues to predict the course of the disease and to foresee personalized treatments in RA patients. © 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license

Gabriele, D. S., Barbara, T., Annalaurafedele,, Gremese, E., Alivernini, S., Chiara, N., Ria, F., Gianfranco, F., Collagen Specific T-Cell Repertoire and HLA-DR Alleles: Biomarkers of Active Refractory Rheumatoid Arthritis, <<EBIOMEDICINE>>, 2015; (Novembre): 2037-2045. [doi:10.1016/j.ebiom.2015.11.019] [http://hdl.handle.net/10807/70469]

Collagen Specific T-Cell Repertoire and HLA-DR Alleles: Biomarkers of Active Refractory Rheumatoid Arthritis

Gabriele, Di Sante;Gremese, Elisa;Alivernini, Stefano;Ria, Francesco;
2015

Abstract

Rheumatoid arthritis (RA) is characterized by chronic joint inflammation and associates with HLA-DRB1*04. The Collagen IIp261-273-specific T cell repertoire in the peripheral blood of DR4+ patients at the onset of the disease shows a restricted TCR-beta chain usage among which the most frequent is TRBV25. To define whether this group of DR4-restricted collagen-specific shared T cell could represent markers of activesevere disease and response to therapy, 90 subjects affected by early-RA were enrolled in the study; peripheral blood mononuclear cells were cultured with or without the human collagen II peptide p261-273 and were examined by immunoscope analysis for the usage of the previously identified shared TCR-beta chains. We report that the presence of T cells carrying rearrangement TRBV25 associated with HLA-DR haplotype and disease activity. HLA-DRB1* haplotypes 04–04, 04–01 and 04–11 were significantly associated with usage of TRBV25, higher disease activity at the onset of disease and poor response to DMARDs. Finally, the HLA-DRB1* haplotype appeared complementary with current serologic tools to predict good and poor responders in a treat to target strategy. The data reported here offer clues to predict the course of the disease and to foresee personalized treatments in RA patients. © 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license
Inglese
Gabriele, D. S., Barbara, T., Annalaurafedele,, Gremese, E., Alivernini, S., Chiara, N., Ria, F., Gianfranco, F., Collagen Specific T-Cell Repertoire and HLA-DR Alleles: Biomarkers of Active Refractory Rheumatoid Arthritis, <<EBIOMEDICINE>>, 2015; (Novembre): 2037-2045. [doi:10.1016/j.ebiom.2015.11.019] [http://hdl.handle.net/10807/70469]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/70469
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