Hemoglobin-based blood substitutes are one of the options available to derive a resuscitating fluid taking into account clinical and physiological demands. In this paper we investigated a novel protein, Hb(αα,ββ) obtained as a combination of two homodimers α2 and β2 both derived from a fusion gene containing two alfa chains or two beta chains respectively coupled via a specific linker. The construct here described is thus a novel heterodimeric hemoglobin carrying four heme groups. The protein cannot dissociate into dimers, as demonstrated by its absence of reactivity versus haptoglobin, and is expected to have a relatively long circulating half-life. The modification does not increase the autoxidation rate, but increases the oxygen affinity, due to a destabilization of the T quaternary state. Characterization of the biochemical properties of this protein in comparison with HbA is reported.

Panetta, G., Arcovito, A., Morea, V., Bellelli, A., Miele, A. E., Hb(αα,ββ): a novel fusion construct for a dimeric, four domain hemoglobin, <<BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS>>, 2008; (10): 1462-1470 [http://hdl.handle.net/10807/6789]

Hb(αα,ββ): a novel fusion construct for a dimeric, four domain hemoglobin

Arcovito, Alessandro;
2008

Abstract

Hemoglobin-based blood substitutes are one of the options available to derive a resuscitating fluid taking into account clinical and physiological demands. In this paper we investigated a novel protein, Hb(αα,ββ) obtained as a combination of two homodimers α2 and β2 both derived from a fusion gene containing two alfa chains or two beta chains respectively coupled via a specific linker. The construct here described is thus a novel heterodimeric hemoglobin carrying four heme groups. The protein cannot dissociate into dimers, as demonstrated by its absence of reactivity versus haptoglobin, and is expected to have a relatively long circulating half-life. The modification does not increase the autoxidation rate, but increases the oxygen affinity, due to a destabilization of the T quaternary state. Characterization of the biochemical properties of this protein in comparison with HbA is reported.
Inglese
Panetta, G., Arcovito, A., Morea, V., Bellelli, A., Miele, A. E., Hb(αα,ββ): a novel fusion construct for a dimeric, four domain hemoglobin, <<BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS>>, 2008; (10): 1462-1470 [http://hdl.handle.net/10807/6789]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/6789
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