Background. Cigarette smoking is implicated in atherosclerotic plaque formation, but the role of nicotine in this process is not completely understood. The release of platelet-derived growth factor (PDGF) by the bovine aortic smooth muscle cell (SMC) after nicotine administration at a concentration similar to that ingested by active and passive smokers and the role of PDGF in SMC cytoskeletal modification were studied. Methods. SMC, harvested with enzymatic digestion from calf aorta, were stimulated in a serum-free medium for 72 hours with (-)-nicotine (from 6 × 10-4 mol/L to 6 × 10-8 mol/L). The release of PDGF was assessed by inhibition antibody-binding assay and confirmed by Western blotting. Mitogenic activity of nicotine on SMCs was also determined. The SMC cytoskeleton was studied with specific antibodies anti-α-actin fibers, anti-vimentin, and anti-β-tubulin, and the modification induced by PDGF was assessed by blocking PDGF activity with specific antibodies. Results. The greatest PDGF release (1.24 ± 0.14 ng/104 cells vs control 0.43 ± 0.07 ng/104 cells) was noted at a (-)-nicotine concentration of 6 × 10-7 mol/L (P < .001). The addition of monoclonal antibody anti-PDGF decreased the tritiated thymidine uptake of SMCs exposed to (-)-nicotine compared with the control (29% vs 5% - P < .001). SMCs exposed to (-)-nicotine concentration of 6 × 10-7 mol/L and 6 × 10-8 mol/L had a significant alteration in the expression of α-actin fibers, vimentin, and β-tubulin compared with control. The administration of antibody anti-PDGF in the culture medium reversed cytoskeletal alteration. Conclusions. Nicotine enhanced the release of platelet-derived growth, which in turn caused an alteration in cytoskeletal organization.

Cucina, A., Sapienza, P., Corvino, V., Borrelli, V., Randone, B., Santoro D'Angelo, L., Cavallaro, A., Nicotine induces platelet-derived growth factor release and cytoskeletal alteration in aortic smooth muscle cells., <<SURGERY>>, 2000; (127(1):): 72-78 [http://hdl.handle.net/10807/6385]

Nicotine induces platelet-derived growth factor release and cytoskeletal alteration in aortic smooth muscle cells.

Corvino, Valentina;
2000

Abstract

Background. Cigarette smoking is implicated in atherosclerotic plaque formation, but the role of nicotine in this process is not completely understood. The release of platelet-derived growth factor (PDGF) by the bovine aortic smooth muscle cell (SMC) after nicotine administration at a concentration similar to that ingested by active and passive smokers and the role of PDGF in SMC cytoskeletal modification were studied. Methods. SMC, harvested with enzymatic digestion from calf aorta, were stimulated in a serum-free medium for 72 hours with (-)-nicotine (from 6 × 10-4 mol/L to 6 × 10-8 mol/L). The release of PDGF was assessed by inhibition antibody-binding assay and confirmed by Western blotting. Mitogenic activity of nicotine on SMCs was also determined. The SMC cytoskeleton was studied with specific antibodies anti-α-actin fibers, anti-vimentin, and anti-β-tubulin, and the modification induced by PDGF was assessed by blocking PDGF activity with specific antibodies. Results. The greatest PDGF release (1.24 ± 0.14 ng/104 cells vs control 0.43 ± 0.07 ng/104 cells) was noted at a (-)-nicotine concentration of 6 × 10-7 mol/L (P < .001). The addition of monoclonal antibody anti-PDGF decreased the tritiated thymidine uptake of SMCs exposed to (-)-nicotine compared with the control (29% vs 5% - P < .001). SMCs exposed to (-)-nicotine concentration of 6 × 10-7 mol/L and 6 × 10-8 mol/L had a significant alteration in the expression of α-actin fibers, vimentin, and β-tubulin compared with control. The administration of antibody anti-PDGF in the culture medium reversed cytoskeletal alteration. Conclusions. Nicotine enhanced the release of platelet-derived growth, which in turn caused an alteration in cytoskeletal organization.
Inglese
Cucina, A., Sapienza, P., Corvino, V., Borrelli, V., Randone, B., Santoro D'Angelo, L., Cavallaro, A., Nicotine induces platelet-derived growth factor release and cytoskeletal alteration in aortic smooth muscle cells., <<SURGERY>>, 2000; (127(1):): 72-78 [http://hdl.handle.net/10807/6385]
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/6385
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact