Abstract BACKGROUND: Breath condensate can give useful information on volatile compounds produced at alveolar level. Actual concentration of H(2)O(2) in breath condensate is dependent on its production at alveolar level and on the efficacy of the detoxifying systems, catalase, glutathione peroxidase, etc. METHODS: In the present paper, a simple chemiluminescent method for the determination of the H(2)O(2) collected in exhaled breath is shown and data of both smokers and nonsmokers volunteers are presented. RESULTS: The chemiluminescent response is linear up to 100 micromol/l H(2)O(2). The analytical sensitivity is about 0.01 micromol/l. Most of the nonsmokers have a H(2)O(2) content lower than 0.05 micromol/l, while smokers have a content ranging from 0.1 to 0.6 micromol/l.

Zappacosta, B., Persichilli, S., Mormile, F., Russo, A., Giardina, B., De Sole, P., A fast chemiluminescent method for H2O2 measurement in exhaled breath condensate., <<CLINICA CHIMICA ACTA>>, 2001; (310): 187-191 [http://hdl.handle.net/10807/62968]

A fast chemiluminescent method for H2O2 measurement in exhaled breath condensate.

Zappacosta, Bruno;Persichilli, Silvia;Mormile, Flaminio;Russo, Andrea;Giardina, Bruno;De Sole, Pasquale
2001

Abstract

Abstract BACKGROUND: Breath condensate can give useful information on volatile compounds produced at alveolar level. Actual concentration of H(2)O(2) in breath condensate is dependent on its production at alveolar level and on the efficacy of the detoxifying systems, catalase, glutathione peroxidase, etc. METHODS: In the present paper, a simple chemiluminescent method for the determination of the H(2)O(2) collected in exhaled breath is shown and data of both smokers and nonsmokers volunteers are presented. RESULTS: The chemiluminescent response is linear up to 100 micromol/l H(2)O(2). The analytical sensitivity is about 0.01 micromol/l. Most of the nonsmokers have a H(2)O(2) content lower than 0.05 micromol/l, while smokers have a content ranging from 0.1 to 0.6 micromol/l.
Inglese
Zappacosta, B., Persichilli, S., Mormile, F., Russo, A., Giardina, B., De Sole, P., A fast chemiluminescent method for H2O2 measurement in exhaled breath condensate., <<CLINICA CHIMICA ACTA>>, 2001; (310): 187-191 [http://hdl.handle.net/10807/62968]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/62968
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