The growth-inhibitory effects of the astaxanthin-rich Haematococcus pluvialis were studied in HCT-116 colon cancer cells. H. pluvialis extract (5 25 μg/ml) inhibited cell growth in a dose- and time-dependent manner, by arresting cell cycle progression and by promoting apoptosis. At 25 μg/ml of H. pluvialis extract, an increase of p53, p21WAF-1/CIP-1 and p27 expression (220%, 160%, 250%, respectively) was observed, concomitantly with a decrease of cyclin D1 expression (58%) and AKT phosphorylation (21%). Moreover, the extract, at the same concentration, strongly up-regulated apoptosis by modifying the ratio of Bax/Bcl-2 and Bcl-XL, and increased the phosphorylation of p38, JNK, and ERK1/2 by 160%, 242%, 280%, respectively. Growth-inhibitory effects by H. pluvialis were also observed in HT-29, LS-174, WiDr, SW-480 cells. This study suggests that H. pluvialis may protect from colon cancer.
Palozza, P., Torelli, C., Boninsegna Lucarelli, A., Simone, R. E., Catalano, A., Mele, M. C., Picci, N., Growth-inhibitory effects of the astaxanthin-rich alga Haematococcus pluvialis in human colon cancer cells., <<CANCER LETTERS>>, 2009; 28 (283): 108-117 [http://hdl.handle.net/10807/5265]
Growth-inhibitory effects of the astaxanthin-rich alga Haematococcus pluvialis in human colon cancer cells.
Palozza, Paola;Boninsegna Lucarelli, Alma;Simone, Rossella Emanuela;Catalano, Assunta;Mele, Maria Cristina;
2009
Abstract
The growth-inhibitory effects of the astaxanthin-rich Haematococcus pluvialis were studied in HCT-116 colon cancer cells. H. pluvialis extract (5 25 μg/ml) inhibited cell growth in a dose- and time-dependent manner, by arresting cell cycle progression and by promoting apoptosis. At 25 μg/ml of H. pluvialis extract, an increase of p53, p21WAF-1/CIP-1 and p27 expression (220%, 160%, 250%, respectively) was observed, concomitantly with a decrease of cyclin D1 expression (58%) and AKT phosphorylation (21%). Moreover, the extract, at the same concentration, strongly up-regulated apoptosis by modifying the ratio of Bax/Bcl-2 and Bcl-XL, and increased the phosphorylation of p38, JNK, and ERK1/2 by 160%, 242%, 280%, respectively. Growth-inhibitory effects by H. pluvialis were also observed in HT-29, LS-174, WiDr, SW-480 cells. This study suggests that H. pluvialis may protect from colon cancer.
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