The Enterococcus faecalis leucine-rich protein ElrA promotes virulence by stimulating bacterial persistence in macrophages and production of the interleukin-6 (IL-6) cytokine. The ElrA protein is encoded within an operon that is poorly expressed under laboratory conditions but induced in vivo. In this study, we identify ef2687 (renamed elrR), which encodes a member of the Rgg (regulator gene for glucosyltransferase) family of putative regulatory proteins. Using quantitative reverse transcription-PCR, translational lacZ fusions, and electrophoretic mobility shift assays, we demonstrate that ElrR positively regulates expression of elrA. These results correlate with the attenuated virulence of the ΔelrR strain in a mouse peritonitis model. Virulence of simple and double elrR and elrA deletion mutants also suggests a remaining ElrR-independent expression of elrA in vivo and additional virulence-related genes controlled by ElrR.

Dumoulin, R., Cortes Perez, N., Gaubert, S., Duhutrel, P., Brinster, S., Torelli, R., Sanguinetti, M., Posteraro, B., Repoila, F., Serror, P., Enterococcal Rgg-Like Regulator ElrR Activates Expression of the elrA Operon, <<JOURNAL OF BACTERIOLOGY>>, 2013; 195 (13): 3073-3083. [doi:10.1128/JB.00121-13] [http://hdl.handle.net/10807/48100]

Enterococcal Rgg-Like Regulator ElrR Activates Expression of the elrA Operon

Torelli, Riccardo;Sanguinetti, Maurizio;Posteraro, Brunella;
2013

Abstract

The Enterococcus faecalis leucine-rich protein ElrA promotes virulence by stimulating bacterial persistence in macrophages and production of the interleukin-6 (IL-6) cytokine. The ElrA protein is encoded within an operon that is poorly expressed under laboratory conditions but induced in vivo. In this study, we identify ef2687 (renamed elrR), which encodes a member of the Rgg (regulator gene for glucosyltransferase) family of putative regulatory proteins. Using quantitative reverse transcription-PCR, translational lacZ fusions, and electrophoretic mobility shift assays, we demonstrate that ElrR positively regulates expression of elrA. These results correlate with the attenuated virulence of the ΔelrR strain in a mouse peritonitis model. Virulence of simple and double elrR and elrA deletion mutants also suggests a remaining ElrR-independent expression of elrA in vivo and additional virulence-related genes controlled by ElrR.
2013
Inglese
Dumoulin, R., Cortes Perez, N., Gaubert, S., Duhutrel, P., Brinster, S., Torelli, R., Sanguinetti, M., Posteraro, B., Repoila, F., Serror, P., Enterococcal Rgg-Like Regulator ElrR Activates Expression of the elrA Operon, <<JOURNAL OF BACTERIOLOGY>>, 2013; 195 (13): 3073-3083. [doi:10.1128/JB.00121-13] [http://hdl.handle.net/10807/48100]
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/48100
Citazioni
  • ???jsp.display-item.citation.pmc??? 10
  • Scopus 11
  • ???jsp.display-item.citation.isi??? 11
social impact