Caveolin-1 (cav-1) is the principal structural component of caveolae which functions as scaffoldiprotein for the integration of a variety of signaling pathways. In this study, we show that siRNA-induced cav-1 down regulation in human endothelial cells (EC) increased cell size and provoked cell cycle arrest at G1/S phase transition. In addition, silencing of cav-1 reduced matrix metalloproteinases (MMPs) activity which, in turn, affected cell migration and VEGF-induced tube formation of EC in vitro. These data indicate that proper expression of cav-1 is required for maintaining typical functions of EC such as proliferation and the formation of new blood vessels. In addition, we observed a marked increase of cell size, after cav-1 silencing, which might indicate the involvement of this scaffolding protein in the way by which cells perceive changes in their microenvironment. In conclusion, this study proposes cav-1 as an interesting target molecule for studying cellular mechanisms which occur in physiological as well as pathological conditions such as senescence and tumorigenesis.

D'Alessio, A., Sica, G., The role of caveolin-1 in the regulation of angiogenesis, Abstract de <<CONGRESSO NAZIONALE DELLA SOCIETA' ITALIANA DI ANATOMIA E ISTOLOGIA (SIAI)>>, (Pistoia, 20-23 September 2012 ), Firenze University Press, Firenze 2012: 53-53 [http://hdl.handle.net/10807/40944]

The role of caveolin-1 in the regulation of angiogenesis

D'Alessio, Alessio;Sica, Gigliola
2012

Abstract

Caveolin-1 (cav-1) is the principal structural component of caveolae which functions as scaffoldiprotein for the integration of a variety of signaling pathways. In this study, we show that siRNA-induced cav-1 down regulation in human endothelial cells (EC) increased cell size and provoked cell cycle arrest at G1/S phase transition. In addition, silencing of cav-1 reduced matrix metalloproteinases (MMPs) activity which, in turn, affected cell migration and VEGF-induced tube formation of EC in vitro. These data indicate that proper expression of cav-1 is required for maintaining typical functions of EC such as proliferation and the formation of new blood vessels. In addition, we observed a marked increase of cell size, after cav-1 silencing, which might indicate the involvement of this scaffolding protein in the way by which cells perceive changes in their microenvironment. In conclusion, this study proposes cav-1 as an interesting target molecule for studying cellular mechanisms which occur in physiological as well as pathological conditions such as senescence and tumorigenesis.
Inglese
ITALIAN JOURNAL OF ANATOMY AND EMBRYOLOGY
CONGRESSO NAZIONALE DELLA SOCIETA' ITALIANA DI ANATOMIA E ISTOLOGIA (SIAI)
Pistoia
20-set-2012
23-set-2012
1122-6714
D'Alessio, A., Sica, G., The role of caveolin-1 in the regulation of angiogenesis, Abstract de <<CONGRESSO NAZIONALE DELLA SOCIETA' ITALIANA DI ANATOMIA E ISTOLOGIA (SIAI)>>, (Pistoia, 20-23 September 2012 ), Firenze University Press, Firenze 2012: 53-53 [http://hdl.handle.net/10807/40944]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/40944
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