The use of the (GTG)5 oligonucleotide, a repetitive marker in the Mycobacterium tuberculosis chromosome, as a primer in association with an IS6110 outlooking primer has been successfully applied to a PCR-based fingerprinting method. This method classified 62 strains of M. tuberculosis, isolated from human immunodeficiency virus-seropositive and -seronegative patients in different regions of Italy and Pakistan, as having 53 different patterns. The results were compared with traditional IS6110 fingerprinting, by which 47 distinct patterns were observed.
Sechi, L., Zanetti, S., Delogu, G., Montinaro, B., Sanna, A., Fadda, G., Molecular epidemiology of Mycobacterium tuberculosis strains isolated from different regions of Italy and Pakistan, <<JOURNAL OF CLINICAL MICROBIOLOGY>>, 1996; 34 (7): 1825-1828 [http://hdl.handle.net/10807/3131]
Molecular epidemiology of Mycobacterium tuberculosis strains isolated from different regions of Italy and Pakistan
Delogu, Giovanni;Fadda, Giovanni
1996
Abstract
The use of the (GTG)5 oligonucleotide, a repetitive marker in the Mycobacterium tuberculosis chromosome, as a primer in association with an IS6110 outlooking primer has been successfully applied to a PCR-based fingerprinting method. This method classified 62 strains of M. tuberculosis, isolated from human immunodeficiency virus-seropositive and -seronegative patients in different regions of Italy and Pakistan, as having 53 different patterns. The results were compared with traditional IS6110 fingerprinting, by which 47 distinct patterns were observed.
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