A challenge in tuberculosis (TB) research is to develop a new immunological test that can help distinguish, among subjects responsive to QuantiFERON TB Gold In tube (QFT-IT), those who are able to control Mtb replication (remote LTBI, recent infection and past TB) from those who cannot (active TB disease). IFN-γ response to the Heparin-binding-hemagglutinin (HBHA) of Mtb has been associated with LTBI, but the cumbersome procedures of purifying the methylated and immunological active form of the protein from Mtb or M. bovis Bacillus Calmette et Guerin (BCG) have prevented its implementation in a diagnostic test. Therefore, the aim of the present study was to evaluate the IFN-γ response to methylated HBHA of Mtb produced in M. smegmatis (rHBHAms) in individuals at different stages of TB who scored positive to QFT-IT.
Delogu, G., Chiacchio, T., Vanini, V., Butera, O., Cuzzi, G., Bua, A., Molicotti, P., Zanetti, S., Lauria, F., Grisetti, S., Magnavita, N., Fadda, G., Girardi, E. G., Goletti, D., Methylated HBHA produced in M. smegmatis discriminates between active and non-active tuberculosis disease among RD1-responders, <<PLOS ONE>>, 2011; 6 (3): e18315-e18315. [doi:10.1371/journal.pone.0018315] [http://hdl.handle.net/10807/31262]
Methylated HBHA produced in M. smegmatis discriminates between active and non-active tuberculosis disease among RD1-responders
Delogu, Giovanni;Bua, Alessandra;Magnavita, Nicola;Fadda, Giovanni;
2011
Abstract
A challenge in tuberculosis (TB) research is to develop a new immunological test that can help distinguish, among subjects responsive to QuantiFERON TB Gold In tube (QFT-IT), those who are able to control Mtb replication (remote LTBI, recent infection and past TB) from those who cannot (active TB disease). IFN-γ response to the Heparin-binding-hemagglutinin (HBHA) of Mtb has been associated with LTBI, but the cumbersome procedures of purifying the methylated and immunological active form of the protein from Mtb or M. bovis Bacillus Calmette et Guerin (BCG) have prevented its implementation in a diagnostic test. Therefore, the aim of the present study was to evaluate the IFN-γ response to methylated HBHA of Mtb produced in M. smegmatis (rHBHAms) in individuals at different stages of TB who scored positive to QFT-IT.
| File | Dimensione | Formato | |
|---|---|---|---|
|
PLosOne_delogu.pdf
accesso aperto
Tipologia file ?:
Versione Editoriale (PDF)
Licenza:
Creative commons
Dimensione
601.53 kB
Formato
Adobe PDF
|
601.53 kB | Adobe PDF | Visualizza/Apri |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
