Leukotriene (LT) B(4) is a potent inflammatory lipid mediator that has been involved in the pathophysiology of respiratory diseases including asthma. Exhaled breath condensate (EBC) is a non-invasive method to sample secretions from the airways. LC/MS/MS techniques for measuring LTB(4) concentrations in EBC have been developed and are suitable for an accurate quantitative assessment of its concentrations in EBC. LC/MS/MS for other eicosanoids including 8-isoprostane, a marker of oxidative stress, and cysteinyl-LTs have been developed. This article, mainly focused on LTB(4), presents the analytical aspects of the LC/MS/MS techniques for measuring LTB(4) and 8-isoprostane in EBC, provides examples of their application to the assessment of airway inflammation in patients with asthma and other respiratory diseases, and discusses their potential utility for non-invasive monitoring of drug therapy.
Montuschi, P., LC/MS/MS analysis of leukotriene B4 in exhaled breath condensate for assessing lung inflammation., <<JOURNAL OF CHROMATOGRAPHY. B>>, 2009; 877 (Maggio): 1272-1280. [doi:10.1016/j.jchromb.2009.01.036] [http://hdl.handle.net/10807/31061]
LC/MS/MS analysis of leukotriene B4 in exhaled breath condensate for assessing lung inflammation.
Montuschi, Paolo
2009
Abstract
Leukotriene (LT) B(4) is a potent inflammatory lipid mediator that has been involved in the pathophysiology of respiratory diseases including asthma. Exhaled breath condensate (EBC) is a non-invasive method to sample secretions from the airways. LC/MS/MS techniques for measuring LTB(4) concentrations in EBC have been developed and are suitable for an accurate quantitative assessment of its concentrations in EBC. LC/MS/MS for other eicosanoids including 8-isoprostane, a marker of oxidative stress, and cysteinyl-LTs have been developed. This article, mainly focused on LTB(4), presents the analytical aspects of the LC/MS/MS techniques for measuring LTB(4) and 8-isoprostane in EBC, provides examples of their application to the assessment of airway inflammation in patients with asthma and other respiratory diseases, and discusses their potential utility for non-invasive monitoring of drug therapy.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.