The large diversity of central nervous system (CNS) tumor types in children and adolescents results in disparate patient outcomes and renders accurate diagnosis challenging. In this study, we prospectively integrated DNA methylation profiling and targeted gene panel sequencing with blinded neuropathological reference diagnostics for a population-based cohort of more than 1,200 newly diagnosed pediatric patients with CNS tumors, to assess their utility in routine neuropathology. We show that the multi-omic integration increased diagnostic accuracy in a substantial proportion of patients through annotation to a refining DNA methylation class (50%), detection of diagnostic or therapeutically relevant genetic alterations (47%) or identification of cancer predisposition syndromes (10%). Discrepant results by neuropathological WHO-based and DNA methylation-based classification (30%) were enriched in histological high-grade gliomas, implicating relevance for current clinical patient management in 5% of all patients. Follow-up (median 2.5 years) suggests improved survival for patients with histological high-grade gliomas displaying lower-grade molecular profiles. These results provide preliminary evidence of the utility of integrating multi-omics in neuropathology for pediatric neuro-oncology.The integration of DNA methylation profiling and targeted sequencing with neuropathology improves the diagnostic accuracy of central nervous system tumors in a population-based cohort of more than 1,200 newly diagnosed pediatric patients.

Sturm, D., Capper, D., Andreiuolo, F., Gessi, M., Kölsche, C., Reinhardt, A., Sievers, P., Wefers, A. K., Ebrahimi, A., Suwala, A. K., Gielen, G. H., Sill, M., Schrimpf, D., Stichel, D., Hovestadt, V., Daenekas, B., Rode, A., Hamelmann, S., Previti, C., Jäger, N., Buchhalter, I., Blattner-Johnson, M., Jones, B. C., Warmuth-Metz, M., Bison, B., Grund, K., Sutter, C., Hirsch, S., Dikow, N., Hasselblatt, M., Schüller, U., Koch, A., Gerber, N. U., White, C. L., Buntine, M. K., Kinross, K., Algar, E. M., Hansford, J. R., Gottardo, N. G., Schuhmann, M. U., Thomale, U. W., Hernáiz Driever, P., Gnekow, A., Witt, O., Müller, H. L., Calaminus, G., Fleischhack, G., Kordes, U., Mynarek, M., Rutkowski, S., Frühwald, M. C., Kramm, C. M., Von Deimling, A., Pietsch, T., Sahm, F., Pfister, S. M., Jones, D. T. W., Multiomic neuropathology improves diagnostic accuracy in pediatric neuro-oncology, <<NATURE MEDICINE>>, 2023; 29 (4): 917-925. [doi:10.1038/s41591-023-02255-1] [https://hdl.handle.net/10807/275062]

Multiomic neuropathology improves diagnostic accuracy in pediatric neuro-oncology

Gessi, Marco
Investigation
;
2023

Abstract

The large diversity of central nervous system (CNS) tumor types in children and adolescents results in disparate patient outcomes and renders accurate diagnosis challenging. In this study, we prospectively integrated DNA methylation profiling and targeted gene panel sequencing with blinded neuropathological reference diagnostics for a population-based cohort of more than 1,200 newly diagnosed pediatric patients with CNS tumors, to assess their utility in routine neuropathology. We show that the multi-omic integration increased diagnostic accuracy in a substantial proportion of patients through annotation to a refining DNA methylation class (50%), detection of diagnostic or therapeutically relevant genetic alterations (47%) or identification of cancer predisposition syndromes (10%). Discrepant results by neuropathological WHO-based and DNA methylation-based classification (30%) were enriched in histological high-grade gliomas, implicating relevance for current clinical patient management in 5% of all patients. Follow-up (median 2.5 years) suggests improved survival for patients with histological high-grade gliomas displaying lower-grade molecular profiles. These results provide preliminary evidence of the utility of integrating multi-omics in neuropathology for pediatric neuro-oncology.The integration of DNA methylation profiling and targeted sequencing with neuropathology improves the diagnostic accuracy of central nervous system tumors in a population-based cohort of more than 1,200 newly diagnosed pediatric patients.
2023
Inglese
Sturm, D., Capper, D., Andreiuolo, F., Gessi, M., Kölsche, C., Reinhardt, A., Sievers, P., Wefers, A. K., Ebrahimi, A., Suwala, A. K., Gielen, G. H., Sill, M., Schrimpf, D., Stichel, D., Hovestadt, V., Daenekas, B., Rode, A., Hamelmann, S., Previti, C., Jäger, N., Buchhalter, I., Blattner-Johnson, M., Jones, B. C., Warmuth-Metz, M., Bison, B., Grund, K., Sutter, C., Hirsch, S., Dikow, N., Hasselblatt, M., Schüller, U., Koch, A., Gerber, N. U., White, C. L., Buntine, M. K., Kinross, K., Algar, E. M., Hansford, J. R., Gottardo, N. G., Schuhmann, M. U., Thomale, U. W., Hernáiz Driever, P., Gnekow, A., Witt, O., Müller, H. L., Calaminus, G., Fleischhack, G., Kordes, U., Mynarek, M., Rutkowski, S., Frühwald, M. C., Kramm, C. M., Von Deimling, A., Pietsch, T., Sahm, F., Pfister, S. M., Jones, D. T. W., Multiomic neuropathology improves diagnostic accuracy in pediatric neuro-oncology, <<NATURE MEDICINE>>, 2023; 29 (4): 917-925. [doi:10.1038/s41591-023-02255-1] [https://hdl.handle.net/10807/275062]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/275062
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