Low-molecular-weight heparins induce decidual heparin-binding epidermal growth factor-like growth factor expression and promote survival of decidual cells undergoing apoptosis

Di Simone, Nicoletta; Di Nicuolo, Fiorella; Castellani, Roberta; Veglia, Manuela; Tersigni, Chiara; Silano, M; Tritarelli, A; Scambia, Giovanni; Marana, Riccardo

doi:10.1016/j.fertnstert.2011.10.021

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Abstract Objective: To evaluate the effects of low-molecular-weight heparins (LMWHs) on decidual heparin-binding epidermal growth factor-like growth factor (HB-EGF) expression/secretion and on TNF-α-induced decidual apoptosis. Design: Experimental study. Setting: Department of Obstetrics and Gynecology, Università Cattolica del Sacro Cuore, Rome, Italy. Patient(s): Cultures of primary decidual cells isolated from human term placenta. Intervention(s): The effects of LMWHs (tinzaparin and enoxaparin) on decidual HB-EGF expression and secretion were investigated by Western blot analysis and ELISA, respectively. TNF-α-induced decidual apoptosis was evaluated by annexin V staining, terminal deoxynucleotide transferase-mediated dUTP nick-end labeling (TUNEL) assay, and caspase activities. Main outcome measure(s): Decidual HB-EGF expression/secretion and apoptotic rate induced by TNF-α were investigated. Result(s): Tinzaparin enhanced decidual HB-EGF expression and secretion. TNF-α reduced the number of viable cells by inducing apoptosis. Simultaneous addition of LMWHs (primarily tinzaparin) blocked the increase in annexin V- and TUNEL-positive cells and reduced the amount of caspase activities. Conclusion(s): Both LMWHs induced a significant increase in decidual HB-EGF expression/secretion and reduced TNF-α-induced decidual apoptosis. Tinzaparin demonstrated higher efficacy.

Di Simone, N., Di Nicuolo, F., Castellani, R., Veglia, M., Tersigni, C., Silano, M., Tritarelli, A., Scambia, G., Marana, R., Low-molecular-weight heparins induce decidual heparin-binding epidermal growth factor-like growth factor expression and promote survival of decidual cells undergoing apoptosis, <<FERTILITY AND STERILITY>>, 2012; (Jan 97(1)): 169-177. [doi:10.1016/j.fertnstert.2011.10.021] [http://hdl.handle.net/10807/26562]

Autori:	Di Simone, Nicoletta Di Nicuolo, Fiorella Castellani, Roberta Veglia, Manuela Tersigni, Chiara Silano, M Tritarelli, A Scambia, Giovanni Marana, Riccardo Mostra autori esterni Nascondi autori esterni
Titolo:	Low-molecular-weight heparins induce decidual heparin-binding epidermal growth factor-like growth factor expression and promote survival of decidual cells undergoing apoptosis
Digital Object Identifier (DOI):	http://dx.doi.org/10.1016/j.fertnstert.2011.10.021
Data di pubblicazione:	2012
Abstract:	Abstract Objective: To evaluate the effects of low-molecular-weight heparins (LMWHs) on decidual heparin-binding epidermal growth factor-like growth factor (HB-EGF) expression/secretion and on TNF-α-induced decidual apoptosis. Design: Experimental study. Setting: Department of Obstetrics and Gynecology, Università Cattolica del Sacro Cuore, Rome, Italy. Patient(s): Cultures of primary decidual cells isolated from human term placenta. Intervention(s): The effects of LMWHs (tinzaparin and enoxaparin) on decidual HB-EGF expression and secretion were investigated by Western blot analysis and ELISA, respectively. TNF-α-induced decidual apoptosis was evaluated by annexin V staining, terminal deoxynucleotide transferase-mediated dUTP nick-end labeling (TUNEL) assay, and caspase activities. Main outcome measure(s): Decidual HB-EGF expression/secretion and apoptotic rate induced by TNF-α were investigated. Result(s): Tinzaparin enhanced decidual HB-EGF expression and secretion. TNF-α reduced the number of viable cells by inducing apoptosis. Simultaneous addition of LMWHs (primarily tinzaparin) blocked the increase in annexin V- and TUNEL-positive cells and reduced the amount of caspase activities. Conclusion(s): Both LMWHs induced a significant increase in decidual HB-EGF expression/secretion and reduced TNF-α-induced decidual apoptosis. Tinzaparin demonstrated higher efficacy.
Lingua:	Inglese
Rivista:	FERTILITY AND STERILITY
Citazione:	Di Simone, N., Di Nicuolo, F., Castellani, R., Veglia, M., Tersigni, C., Silano, M., Tritarelli, A., Scambia, G., Marana, R., Low-molecular-weight heparins induce decidual heparin-binding epidermal growth factor-like growth factor expression and promote survival of decidual cells undergoing apoptosis, <<FERTILITY AND STERILITY>>, 2012; (Jan 97(1)): 169-177. [doi:10.1016/j.fertnstert.2011.10.021] [http://hdl.handle.net/10807/26562]
Appare nelle tipologie:	Articolo in rivista, Nota a sentenza

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