This study reports the detection of a set of 35 peptide fragments and 7 intact proteins in the vitreous humour using a top-down proteomic platform based on high-resolution HPLC-MS and MS/MS analysis. The concentrations of thymosin b4 (R = 0.932) and two peptide fragments (i.e., vimentin fragment (Fr.) 443-465 (R = 0.998) and polyubiquitin Fr. 1-73 (R = 0.968)) were observed to have very strong linear correlations with postmortem intervals. Data are available via ProteomeXchange with identifier PXD037095. These preliminary results suggest that some biochemical molecular events are linearly related to the postmortem interval and that the concentrations of these peptides and fragments can be clinically useful in establishing the time of death if measured within the first 160 h postmortem. (c) 2022 Elsevier B.V. All rights reserved.
Boroumand, M., Grassi, V. M., Castagnola, F., De-Giorgio, F., D'Aloja, E., Vetrugno, G., Pascali, V. L., Vincenzoni, F., Iavarone, F., Faa, G., Castagnola, M., Estimation of postmortem interval using top-down HPLC–MS analysis of peptide fragments in vitreous humour: A pilot study, <<INTERNATIONAL JOURNAL OF MASS SPECTROMETRY>>, 2023; 483 (5): N/A-N/A. [doi:10.1016/j.ijms.2022.116952] [https://hdl.handle.net/10807/262300]
Estimation of postmortem interval using top-down HPLC–MS analysis of peptide fragments in vitreous humour: A pilot study
De-Giorgio, F.;Vetrugno, Giuseppe;Pascali, Vincenzo Lorenzo;Iavarone, Federica;Castagnola, Massimo
2023
Abstract
This study reports the detection of a set of 35 peptide fragments and 7 intact proteins in the vitreous humour using a top-down proteomic platform based on high-resolution HPLC-MS and MS/MS analysis. The concentrations of thymosin b4 (R = 0.932) and two peptide fragments (i.e., vimentin fragment (Fr.) 443-465 (R = 0.998) and polyubiquitin Fr. 1-73 (R = 0.968)) were observed to have very strong linear correlations with postmortem intervals. Data are available via ProteomeXchange with identifier PXD037095. These preliminary results suggest that some biochemical molecular events are linearly related to the postmortem interval and that the concentrations of these peptides and fragments can be clinically useful in establishing the time of death if measured within the first 160 h postmortem. (c) 2022 Elsevier B.V. All rights reserved.
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