PDGFRB, a transmembrane tyrosine kinase receptor for platelet-derived growth factor, is constitutively activated by gene fusion with different partners in myeloproliferative/myelodysplastic disorders with peculiar clinical characteristics. Six alternative partner genes have been described thus far. In this study, we report the molecular cloning of a novel translocation t(5;17)(q33;p11.2) in a case of juvenile myelomonocytic leukemia. The novel partner gene was identified as HCMOGT-1 using 5'-rapid amplification of cDNA ends; fluorescence in situ hybridization and reverse transcriptase-PCR analyses confirmed that the translocation resulted in PDGFRB/HCMOGT-1 fusion. We show that the breakpoint of PDGFRB occurred at the same site of all previously reported PDGFRB translocations.

Morerio, C., Acquila, M., Rosanda, C., Rapella, A., Dufour, C., Locatelli, F., Maserati, E., Pasquali, F., Panarello, C., HCMOGT-1 is a novel fusion partner to PDGFRB in juvenile myelomonocytic leukemia with t(5;17)(q33;p11.2), <<CANCER RESEARCH>>, 2004; 64 (8): 2649-2651. [doi:10.1158/0008-5472.can-03-4026] [https://hdl.handle.net/10807/260456]

HCMOGT-1 is a novel fusion partner to PDGFRB in juvenile myelomonocytic leukemia with t(5;17)(q33;p11.2)

Locatelli, Franco;
2004

Abstract

PDGFRB, a transmembrane tyrosine kinase receptor for platelet-derived growth factor, is constitutively activated by gene fusion with different partners in myeloproliferative/myelodysplastic disorders with peculiar clinical characteristics. Six alternative partner genes have been described thus far. In this study, we report the molecular cloning of a novel translocation t(5;17)(q33;p11.2) in a case of juvenile myelomonocytic leukemia. The novel partner gene was identified as HCMOGT-1 using 5'-rapid amplification of cDNA ends; fluorescence in situ hybridization and reverse transcriptase-PCR analyses confirmed that the translocation resulted in PDGFRB/HCMOGT-1 fusion. We show that the breakpoint of PDGFRB occurred at the same site of all previously reported PDGFRB translocations.
2004
Inglese
Morerio, C., Acquila, M., Rosanda, C., Rapella, A., Dufour, C., Locatelli, F., Maserati, E., Pasquali, F., Panarello, C., HCMOGT-1 is a novel fusion partner to PDGFRB in juvenile myelomonocytic leukemia with t(5;17)(q33;p11.2), <<CANCER RESEARCH>>, 2004; 64 (8): 2649-2651. [doi:10.1158/0008-5472.can-03-4026] [https://hdl.handle.net/10807/260456]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/260456
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