We investigated immune reconstitution against human cytornegalovirus (HCMV) in 57 hematopoietic stem cell transplant (HSCT) recipients, aged 1 to 24 years, through a novel method combining T-cell stimulation by HCMV-infected autologous dendritic cells with simultaneous cytometric quantification of HCMV-specific, IFN gamma-producing CD4(+) and CD8(+) T cells. Lymphoproliferative response (LPR) to HCMV antigens was also determined. Patients were stratified into 2 groups according to HCMV serostatus, comprising 39 HCMV-seropositive (R+) and 18 HCMV-seronegative (R-) patients who received a transplant from a seropositive donor. Recovery of both HCMV-specific CD4(+) and CD8(+) T-cell immunity occurred in all 39 R+ patients within 6 months and in 6 (33%) of 18 R- patients within 12 months. In R+ patients, the median numbers of HCMV-specific CD8(+) and CD4(+) Tcells were significantly higher than those of healthy controls, starting from days +60 and +180, respectively. In R- patients, the median numbers of HCMV-specific T cells were consistently lower than in R+ patients. LPR was delayed compared with reconstitution of IFN gamma-producing T cells. Patients with delayed specific immune reconstitution experienced recurrent episodes of HCMV infection. HCMV seropositivity of young HSCT recipients is the major factor responsible for HCMV-specific immune reconstitution, irrespective of donor serostatus, and measurement of HCMV-specific T cells appears useful for correct management of HCMV infection.

Lilleri, D., Gerna, G., Fornara, C., Lozza, L., Maccario, R., Locatelli, F., Prospective simultaneous quantification of human cytomegalovirus-specific CD4+ and CD8+ T-cell reconstitution in young recipients of allogeneic hematopoietic stem cell transplants, <<BLOOD>>, 2006; 108 (4): 1406-1412. [doi:10.1182/blood-2005-11-012864] [https://hdl.handle.net/10807/259038]

Prospective simultaneous quantification of human cytomegalovirus-specific CD4+ and CD8+ T-cell reconstitution in young recipients of allogeneic hematopoietic stem cell transplants

Locatelli, Franco
2006

Abstract

We investigated immune reconstitution against human cytornegalovirus (HCMV) in 57 hematopoietic stem cell transplant (HSCT) recipients, aged 1 to 24 years, through a novel method combining T-cell stimulation by HCMV-infected autologous dendritic cells with simultaneous cytometric quantification of HCMV-specific, IFN gamma-producing CD4(+) and CD8(+) T cells. Lymphoproliferative response (LPR) to HCMV antigens was also determined. Patients were stratified into 2 groups according to HCMV serostatus, comprising 39 HCMV-seropositive (R+) and 18 HCMV-seronegative (R-) patients who received a transplant from a seropositive donor. Recovery of both HCMV-specific CD4(+) and CD8(+) T-cell immunity occurred in all 39 R+ patients within 6 months and in 6 (33%) of 18 R- patients within 12 months. In R+ patients, the median numbers of HCMV-specific CD8(+) and CD4(+) Tcells were significantly higher than those of healthy controls, starting from days +60 and +180, respectively. In R- patients, the median numbers of HCMV-specific T cells were consistently lower than in R+ patients. LPR was delayed compared with reconstitution of IFN gamma-producing T cells. Patients with delayed specific immune reconstitution experienced recurrent episodes of HCMV infection. HCMV seropositivity of young HSCT recipients is the major factor responsible for HCMV-specific immune reconstitution, irrespective of donor serostatus, and measurement of HCMV-specific T cells appears useful for correct management of HCMV infection.
2006
Inglese
Lilleri, D., Gerna, G., Fornara, C., Lozza, L., Maccario, R., Locatelli, F., Prospective simultaneous quantification of human cytomegalovirus-specific CD4+ and CD8+ T-cell reconstitution in young recipients of allogeneic hematopoietic stem cell transplants, <<BLOOD>>, 2006; 108 (4): 1406-1412. [doi:10.1182/blood-2005-11-012864] [https://hdl.handle.net/10807/259038]
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/259038
Citazioni
  • ???jsp.display-item.citation.pmc??? 24
  • Scopus 87
  • ???jsp.display-item.citation.isi??? 85
social impact