Graft-versus-host disease (GvHD) is the main complication after haematopoietic stem cells transplantation (HSCT) and acute forms (aGvHD) occur in 20-40% of cases even after donor (D) and recipient (R) HLA matching, apparently because of D/R minor histocompatibility antigen (mHA) mismatches and cytokine polymorphisms. The genotype of cytokines and mHA of 77 haematological R following HSCT from HLA identical siblings were determined to detect genetic polymorphisms correlated with GvHD. We analysed TNFA (-863 C/A, -857 C/T and G/A at positions -574, -376, -308, -244, -238), IL-10 (-1082 G/A, -819 C/A, -592 C/T), IL-1B (T/C +3953), IL-1RA (VNTR), HA-1 (H/R allele) and CD-31 (C/G at codon 125, A/G at codon 563). Allele frequencies were in Hardy-Weinberg equilibrium and similar to those of 77 healthy controls. We observed positive correlations between a lower risk of clinically significant aGvHD and both the presence of -1082G -819C -592C IL-10 haplotype when both R and D are considered together and the absence of R IL-1RA allele 2. Furthermore, we observed an association between the absence of TNF-A -238 A allele and the risk of extensive chronic GvHD. mHA and cytokines genotyping would thus seem a valid source of information for the prior identification of recipients with a higher risk of aGvHD.

Bertinetto, F. E., Dall'Omo, A. M., Mazzola, G. A., Rendine, S., Berrino, M., Bertola, L., Magistroni, P., Caropreso, P., Falda, M., Locatelli, F., Busca, A., Amoroso, A., Role of non-HLA genetic polymorphisms in graft-versus-host disease after haematopoietic stem cell transplantation, <<INTERNATIONAL JOURNAL OF IMMUNOGENETICS>>, 2006; 33 (5): 375-384. [doi:10.1111/j.1744-313X.2006.00630.x] [https://hdl.handle.net/10807/258978]

Role of non-HLA genetic polymorphisms in graft-versus-host disease after haematopoietic stem cell transplantation

Locatelli, Franco;
2006

Abstract

Graft-versus-host disease (GvHD) is the main complication after haematopoietic stem cells transplantation (HSCT) and acute forms (aGvHD) occur in 20-40% of cases even after donor (D) and recipient (R) HLA matching, apparently because of D/R minor histocompatibility antigen (mHA) mismatches and cytokine polymorphisms. The genotype of cytokines and mHA of 77 haematological R following HSCT from HLA identical siblings were determined to detect genetic polymorphisms correlated with GvHD. We analysed TNFA (-863 C/A, -857 C/T and G/A at positions -574, -376, -308, -244, -238), IL-10 (-1082 G/A, -819 C/A, -592 C/T), IL-1B (T/C +3953), IL-1RA (VNTR), HA-1 (H/R allele) and CD-31 (C/G at codon 125, A/G at codon 563). Allele frequencies were in Hardy-Weinberg equilibrium and similar to those of 77 healthy controls. We observed positive correlations between a lower risk of clinically significant aGvHD and both the presence of -1082G -819C -592C IL-10 haplotype when both R and D are considered together and the absence of R IL-1RA allele 2. Furthermore, we observed an association between the absence of TNF-A -238 A allele and the risk of extensive chronic GvHD. mHA and cytokines genotyping would thus seem a valid source of information for the prior identification of recipients with a higher risk of aGvHD.
2006
Inglese
Bertinetto, F. E., Dall'Omo, A. M., Mazzola, G. A., Rendine, S., Berrino, M., Bertola, L., Magistroni, P., Caropreso, P., Falda, M., Locatelli, F., Busca, A., Amoroso, A., Role of non-HLA genetic polymorphisms in graft-versus-host disease after haematopoietic stem cell transplantation, <<INTERNATIONAL JOURNAL OF IMMUNOGENETICS>>, 2006; 33 (5): 375-384. [doi:10.1111/j.1744-313X.2006.00630.x] [https://hdl.handle.net/10807/258978]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/258978
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