Mesenchymal stromal cells (MSCs) are adult multipotent cells that can be isolated from several human tissues. MSCs represent a novel and attractive tool in strategies of cellular therapy. For in vivo use, MSCs have to be ex vivo expanded in order to reach the numbers suitable for their clinical application. Despite being efficacious, the use of fetal calf serum for MSC ex vivo expansion for clinical purposes raises concerns related to immunization and transmission of zoonoses; the standardization of expansion methods, possibly devoid of animal components, such as those based on platelet lysate, are discussed in this paper. Moreover, this review focuses on the search of novel markers for the prospective identification/isolation of MSCs and on the potential risks connected with ex vivo expansion of MSCs, in particular that of their malignant transformation. Available tests to study the genetic stability of ex vivo expanded MSCs are also analyzed. (C) 2010 Elsevier Ltd. All rights reserved.

Bernardo, M. E., Cometa, A. M., Pagliara, D., Vinti, L., Rossi, F., Cristantielli, R., Palumbo, G., Locatelli, F., Ex vivo expansion of mesenchymal stromal cells, <<BAILLIERE'S BEST PRACTICE IN CLINICAL HAEMATOLOGY>>, 2011; 24 (1): 73-81. [doi:10.1016/j.beha.2010.11.002] [https://hdl.handle.net/10807/249174]

Ex vivo expansion of mesenchymal stromal cells

Locatelli, Franco
2011

Abstract

Mesenchymal stromal cells (MSCs) are adult multipotent cells that can be isolated from several human tissues. MSCs represent a novel and attractive tool in strategies of cellular therapy. For in vivo use, MSCs have to be ex vivo expanded in order to reach the numbers suitable for their clinical application. Despite being efficacious, the use of fetal calf serum for MSC ex vivo expansion for clinical purposes raises concerns related to immunization and transmission of zoonoses; the standardization of expansion methods, possibly devoid of animal components, such as those based on platelet lysate, are discussed in this paper. Moreover, this review focuses on the search of novel markers for the prospective identification/isolation of MSCs and on the potential risks connected with ex vivo expansion of MSCs, in particular that of their malignant transformation. Available tests to study the genetic stability of ex vivo expanded MSCs are also analyzed. (C) 2010 Elsevier Ltd. All rights reserved.
2011
Inglese
Bernardo, M. E., Cometa, A. M., Pagliara, D., Vinti, L., Rossi, F., Cristantielli, R., Palumbo, G., Locatelli, F., Ex vivo expansion of mesenchymal stromal cells, <<BAILLIERE'S BEST PRACTICE IN CLINICAL HAEMATOLOGY>>, 2011; 24 (1): 73-81. [doi:10.1016/j.beha.2010.11.002] [https://hdl.handle.net/10807/249174]
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/249174
Citazioni
  • ???jsp.display-item.citation.pmc??? 41
  • Scopus 79
  • ???jsp.display-item.citation.isi??? 72
social impact