Optimization of phenylthiohydantion (PTH)-amino acid separation by micellar electrokinetic capillary chromatography was achieved by the use of a weighted variable-size simplex algorithm. The optimization procedure concerned the pH of the aqueous buffer, the sodium dodecyl sulphate concentration and the percentage of organic solvent; the organic solvent used was either methanol or acetonitrile. In both instances the optimization procedure led to very similar final experimental conditions and migration order and times of the PTH-amino acids, showing that the organic solvent probably provides a better polydispersity of the micellar phase. The elution pattern observed in the two instances suggests that ionic interactions and polar repartition play a role in the separation mechanism, but other types of interaction cannot be excluded.

Castagnola, M., Rossetti, D. V., Cassiano, L., Rabino, R., Nocca, G., Giardina, B., OPTIMIZATION OF PHENYLTHIOHYDANTOINAMINO ACID SEPARATION BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY, <<JOURNAL OF CHROMATOGRAPHY A>>, 1993; 638 (2): 327-334. [doi:10.1016/0021-9673(93)83445-X] [http://hdl.handle.net/10807/23608]

OPTIMIZATION OF PHENYLTHIOHYDANTOINAMINO ACID SEPARATION BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY

Castagnola, Massimo;Rossetti, Diana Valeria;Cassiano, Loredana;Nocca, Giuseppina;Giardina, Bruno
1993

Abstract

Optimization of phenylthiohydantion (PTH)-amino acid separation by micellar electrokinetic capillary chromatography was achieved by the use of a weighted variable-size simplex algorithm. The optimization procedure concerned the pH of the aqueous buffer, the sodium dodecyl sulphate concentration and the percentage of organic solvent; the organic solvent used was either methanol or acetonitrile. In both instances the optimization procedure led to very similar final experimental conditions and migration order and times of the PTH-amino acids, showing that the organic solvent probably provides a better polydispersity of the micellar phase. The elution pattern observed in the two instances suggests that ionic interactions and polar repartition play a role in the separation mechanism, but other types of interaction cannot be excluded.
Inglese
Castagnola, M., Rossetti, D. V., Cassiano, L., Rabino, R., Nocca, G., Giardina, B., OPTIMIZATION OF PHENYLTHIOHYDANTOINAMINO ACID SEPARATION BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY, <<JOURNAL OF CHROMATOGRAPHY A>>, 1993; 638 (2): 327-334. [doi:10.1016/0021-9673(93)83445-X] [http://hdl.handle.net/10807/23608]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/23608
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