Somatic cell nuclear transfer (SCNT) was first developed in livestock for the purpose of accelerating the widespread use of superior genotypes. Although many problems still exist now after fifteen years of research owing to the limited understanding of genome reprogramming, SCNT has provided a powerful tool to make copies of selected individuals in different species, to study genome pluripotency and differentiation, opening new avenues of research in regenerative medicine and representing the main route for making transgenic livestock. Besides well-established methods to deliver transgenes, recent development in enzymatic engineering to edit the genome provides more precise and reproducible tools to target-specific genomic loci especially for producing knockout animals. The interest in generating transgenic livestock lies in the agricultural and biomedical areas and it is, in most cases, at the stage of research and development, with few exceptions that are making the way into practical applications.

Galli, C., Lagutina, I., Perota, A., Colleoni, S., Duchi, R., Lucchini, F., Lazzari, G., Somatic cell nuclear transfer and transgenesis in large animals: current and future insights, <<REPRODUCTION IN DOMESTIC ANIMALS>>, 2012; 47 Suppl 3 (Giugno): 2-11. [doi:10.1111/j.1439-0531.2012.02045.x] [http://hdl.handle.net/10807/23190]

Somatic cell nuclear transfer and transgenesis in large animals: current and future insights

Lucchini, Franco;
2012

Abstract

Somatic cell nuclear transfer (SCNT) was first developed in livestock for the purpose of accelerating the widespread use of superior genotypes. Although many problems still exist now after fifteen years of research owing to the limited understanding of genome reprogramming, SCNT has provided a powerful tool to make copies of selected individuals in different species, to study genome pluripotency and differentiation, opening new avenues of research in regenerative medicine and representing the main route for making transgenic livestock. Besides well-established methods to deliver transgenes, recent development in enzymatic engineering to edit the genome provides more precise and reproducible tools to target-specific genomic loci especially for producing knockout animals. The interest in generating transgenic livestock lies in the agricultural and biomedical areas and it is, in most cases, at the stage of research and development, with few exceptions that are making the way into practical applications.
Inglese
http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0531.2012.02045.x/pdf
Galli, C., Lagutina, I., Perota, A., Colleoni, S., Duchi, R., Lucchini, F., Lazzari, G., Somatic cell nuclear transfer and transgenesis in large animals: current and future insights, <>, 2012; 47 Suppl 3 (Giugno): 2-11. [doi:10.1111/j.1439-0531.2012.02045.x] [http://hdl.handle.net/10807/23190]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10807/23190
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