CAR-NK cells may represent a valuable tool, complementary to CART cells, in adoptive immunotherapy of leukemia and solid tumors. However, gene transfer to human NK cells is a challenging task, particularly with non-virus-based techniques. Here, we describe a new procedure allowing efficient electroporation-based transfection of plasmid DNA, including CAR and CCR7 genes, in resting or cytokine-expanded human NK cell populations and NK-92 cell line. This procedure may offer a suitable platform for a safe and effective use of CAR-NK cells in adoptive immunotherapy of cancer.
Ingegnere, T., Mariotti, F. R., Pelosi, A., Quintarelli, C., De Angelis, B., Tumino, N., Besi, F., Cantoni, C., Locatelli, F., Vacca, P., Moretta, L., Human CAR NK cells: A new non-viral method allowing high efficient transfection and strong tumor cell killing, <<FRONTIERS IN IMMUNOLOGY>>, 2019; 10 (APR): 1-10. [doi:10.3389/fimmu.2019.00957] [https://hdl.handle.net/10807/229474]
Human CAR NK cells: A new non-viral method allowing high efficient transfection and strong tumor cell killing
Locatelli, FrancoWriting – Review & Editing
;
2019
Abstract
CAR-NK cells may represent a valuable tool, complementary to CART cells, in adoptive immunotherapy of leukemia and solid tumors. However, gene transfer to human NK cells is a challenging task, particularly with non-virus-based techniques. Here, we describe a new procedure allowing efficient electroporation-based transfection of plasmid DNA, including CAR and CCR7 genes, in resting or cytokine-expanded human NK cell populations and NK-92 cell line. This procedure may offer a suitable platform for a safe and effective use of CAR-NK cells in adoptive immunotherapy of cancer.
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