To investigate seminal antioxidant systems and their correlation with hormonal pattern in varicocele patients, we studied 33 varicocele (VAR) patients (12 oligozoospermic, 21 normozoospermic) and 34 non-VARs (10 idiopathic oligozoospermic, 24 normozoospermic). Non-enzymatic total antioxidant capacity (TAC) was measured using H(2)O(2)-metamyoglobin, which generates the radical form, spectroscopically detectable, of the chromogen 2,2',-azinobis-3-ethylbenzothiazoline-6-sulphonate (ABTS): time till appearance (Lag) of ABTS*(-) signifies antioxidant concentration. Lag was significantly longer in VARs than controls suggesting ineffective utilization of antioxidants. A significant direct correlation (r = 0.65, p < 0.01) of Lag with sperm count was observed in non-VARs, while in VARs it was inverted, as well as with hematic FSH levels. A multivariate analysis including FSH, Lag, progressive spermatozoa, oligozoospermia and varicocele indicated a strong inverse correlation between FSH and motility (r(2) = 0.31, p > F = 0.0007), not modified by Lag (r(2) = 0.31, p > F = 0.002). Their inverse correlation with Lag may suggest that higher FSH levels, improving sperm antioxidant efficiency, counterbalance varicocele-induced dyspermia.

Mancini, A., Milardi, D., Bianchi, A., Festa, R., Silvestrini, A., De Marinis, L., Pontecorvi, A., Meucci, E., Increased total antioxidant capacity in seminal plasma of varicocele patients: a multivariate analysis, <<ARCHIVES OF ANDROLOGY>>, 2007; 53 (1): 37-42. [doi:10.1080/01485010600840756] [http://hdl.handle.net/10807/22547]

Increased total antioxidant capacity in seminal plasma of varicocele patients: a multivariate analysis

Milardi, Domenico;Bianchi, Antonio;Silvestrini, Andrea;De Marinis, Laura;Pontecorvi, Alfredo;Meucci, Elisabetta
2007

Abstract

To investigate seminal antioxidant systems and their correlation with hormonal pattern in varicocele patients, we studied 33 varicocele (VAR) patients (12 oligozoospermic, 21 normozoospermic) and 34 non-VARs (10 idiopathic oligozoospermic, 24 normozoospermic). Non-enzymatic total antioxidant capacity (TAC) was measured using H(2)O(2)-metamyoglobin, which generates the radical form, spectroscopically detectable, of the chromogen 2,2',-azinobis-3-ethylbenzothiazoline-6-sulphonate (ABTS): time till appearance (Lag) of ABTS*(-) signifies antioxidant concentration. Lag was significantly longer in VARs than controls suggesting ineffective utilization of antioxidants. A significant direct correlation (r = 0.65, p < 0.01) of Lag with sperm count was observed in non-VARs, while in VARs it was inverted, as well as with hematic FSH levels. A multivariate analysis including FSH, Lag, progressive spermatozoa, oligozoospermia and varicocele indicated a strong inverse correlation between FSH and motility (r(2) = 0.31, p > F = 0.0007), not modified by Lag (r(2) = 0.31, p > F = 0.002). Their inverse correlation with Lag may suggest that higher FSH levels, improving sperm antioxidant efficiency, counterbalance varicocele-induced dyspermia.
Inglese
Mancini, A., Milardi, D., Bianchi, A., Festa, R., Silvestrini, A., De Marinis, L., Pontecorvi, A., Meucci, E., Increased total antioxidant capacity in seminal plasma of varicocele patients: a multivariate analysis, <<ARCHIVES OF ANDROLOGY>>, 2007; 53 (1): 37-42. [doi:10.1080/01485010600840756] [http://hdl.handle.net/10807/22547]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10807/22547
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