As part of an ongoing study to determine the true habitat of Bacillus species, we report here the isolation and characterisation of Bacillus subtilis from the human gastrointestinal tract (GIT). Strains were obtained from ileum biopsies as well as from faecal samples and their biotypes defined. 16S rRNA analysis revealed that most isolates of B. subtilis were highly conserved, in contrast to RAPD-PCR fingerprinting that showed greater diversity with 23 distinct RAPD types. The majority of B. subtilis strains examined possessed features that could be advantageous to survival within the GIT. This included the ability to form biofilms, to sporulate anaerobically and secretion of antimicrobials. At least one isolate was shown to form spores that carried an exosporium, a loosely attached outer layer to the mature endospore, this being the first report of B. subtilis spores carrying an exosporium. This study reinforces a growing view that B. subtilis and probably other species have adapted to life within the GIT and should be considered gut commensals rather than solely soil microorganisms.
Hong, H., Khaneja, R., Tam, N., Cazzato, I. A., Tan, S., Urdaci, M., Brisson, A., Gasbarrini, A., Barnes, I., Cutting, S., ag) Bacillus subtilis isolated from the human gastrointestinal tract., <<RESEARCH IN MICROBIOLOGY>>, 2008; 2009 (160): 134-143 [http://hdl.handle.net/10807/21645]
ag) Bacillus subtilis isolated from the human gastrointestinal tract.
Cazzato, Immacolata Alessia;Gasbarrini, Antonio;
2009
Abstract
As part of an ongoing study to determine the true habitat of Bacillus species, we report here the isolation and characterisation of Bacillus subtilis from the human gastrointestinal tract (GIT). Strains were obtained from ileum biopsies as well as from faecal samples and their biotypes defined. 16S rRNA analysis revealed that most isolates of B. subtilis were highly conserved, in contrast to RAPD-PCR fingerprinting that showed greater diversity with 23 distinct RAPD types. The majority of B. subtilis strains examined possessed features that could be advantageous to survival within the GIT. This included the ability to form biofilms, to sporulate anaerobically and secretion of antimicrobials. At least one isolate was shown to form spores that carried an exosporium, a loosely attached outer layer to the mature endospore, this being the first report of B. subtilis spores carrying an exosporium. This study reinforces a growing view that B. subtilis and probably other species have adapted to life within the GIT and should be considered gut commensals rather than solely soil microorganisms.
| Campo DC | Valore | Lingua |
|---|---|---|
| dc.authority.academicField2000 | Settore MED/12 - GASTROENTEROLOGIA | it |
| dc.authority.ancejournal | RESEARCH IN MICROBIOLOGY | it |
| dc.authority.erc2011 | Health services, health care research | it |
| dc.authority.people | Hong, Ha | - |
| dc.authority.people | Khaneja, R | - |
| dc.authority.people | Tam, Nm | - |
| dc.authority.people | Cazzato, Immacolata Alessia | it |
| dc.authority.people | Tan, S | - |
| dc.authority.people | Urdaci, M | - |
| dc.authority.people | Brisson, A | - |
| dc.authority.people | Gasbarrini, Antonio | it |
| dc.authority.people | Barnes, I | - |
| dc.authority.people | Cutting, Sm | - |
| dc.cilea.flagannuariono | 3246 | - |
| dc.cilea.flagannuariosi | 7945 | - |
| dc.cilea.flagppdno | 3246 | - |
| dc.cilea.flagppdsi | 7945 | - |
| dc.collection.id.s | e309db74-00b3-0599-e053-3705fe0a55db | * |
| dc.collection.name | Articolo in rivista, Nota a sentenza | * |
| dc.contributor.appartenenza | ROMA - Dipartimento di Medicina e chirurgia traslazionale | * |
| dc.contributor.appartenenza.mi | 33183 | * |
| dc.contributor.faculty | FACOLTA' DI MEDICINA E CHIRURGIA "A.GEMELLI" | * |
| dc.date.accessioned | 2012/07/07 11:47:44 | - |
| dc.date.available | 2012/07/07 11:47:44 | - |
| dc.date.issued | 2009 | - |
| dc.description.abstracteng | As part of an ongoing study to determine the true habitat of Bacillus species, we report here the isolation and characterisation of Bacillus subtilis from the human gastrointestinal tract (GIT). Strains were obtained from ileum biopsies as well as from faecal samples and their biotypes defined. 16S rRNA analysis revealed that most isolates of B. subtilis were highly conserved, in contrast to RAPD-PCR fingerprinting that showed greater diversity with 23 distinct RAPD types. The majority of B. subtilis strains examined possessed features that could be advantageous to survival within the GIT. This included the ability to form biofilms, to sporulate anaerobically and secretion of antimicrobials. At least one isolate was shown to form spores that carried an exosporium, a loosely attached outer layer to the mature endospore, this being the first report of B. subtilis spores carrying an exosporium. This study reinforces a growing view that B. subtilis and probably other species have adapted to life within the GIT and should be considered gut commensals rather than solely soil microorganisms. | - |
| dc.description.allpeople | Hong, Ha; Khaneja, R; Tam, Nm; Cazzato, Immacolata Alessia; Tan, S; Urdaci, M; Brisson, A; Gasbarrini, Antonio; Barnes, I; Cutting, Sm | - |
| dc.description.allpeopleoriginal | Hong, Ha; Khaneja, R; Tam, Nm; Cazzato, Immacolata Alessia; Tan, S; Urdaci, M; Brisson, A; Gasbarrini, Antonio; Barnes, I; Cutting, Sm | it |
| dc.description.fulltext | none | en |
| dc.description.fulltextoriginal | none | en |
| dc.description.languageisokeywords | eng | it |
| dc.description.numberofauthors | 10 | - |
| dc.identifier.citation | Hong, H., Khaneja, R., Tam, N., Cazzato, I. A., Tan, S., Urdaci, M., Brisson, A., Gasbarrini, A., Barnes, I., Cutting, S., ag) Bacillus subtilis isolated from the human gastrointestinal tract., < |
it |
| dc.identifier.pmid | 19068230 | - |
| dc.identifier.uri | http://hdl.handle.net/10807/21645 | - |
| dc.language.iso | eng | it |
| dc.publisher.country | ITA | it |
| dc.relation.firstpage | 134 | it |
| dc.relation.issue | 160 | it |
| dc.relation.issueyear | 2008 | it |
| dc.relation.lastpage | 143 | it |
| dc.relation.numberofpages | 10 | it |
| dc.relation.volume | 2009 | it |
| dc.subject.keywords | Bacillus subtilis | it |
| dc.subject.singlekeyword | Bacillus subtilis | * |
| dc.title | ag) Bacillus subtilis isolated from the human gastrointestinal tract. | it |
| dc.type | art_per_29 | - |
| dc.type.contribution | Articolo su rivista scientifica / specializzata | it |
| dc.type.driver | info:eu-repo/semantics/article | - |
| dc.type.full | 03. Contributo in rivista::Articolo in rivista, Nota a sentenza | it |
| dc.type.genius | Pubblicazione su rivista con Impact Factor | - |
| dc.type.miur | 262 | en |
| dc.type.miurarticle | Articolo in rivista | it |
| dc.type.research | AREA06 - SCIENZE MEDICHE | - |
| Appare nelle tipologie: | Articolo in rivista, Nota a sentenza | |
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