Clinical and experimental evidence indicates that the hepatitis C virus (HCV) E2 glycoprotein (HCV/E2) is the most promising candidate for the development of an effective anti-HCV vaccine. Identification of the human epitopes that are conserved among isolates and are able to elicit protective antibodies would constitute a significant step forward. This work describes the mapping of the B-cell epitopes present on the surface of HCV/E2, as recognized by the immune system during infection, by the analysis of the reciprocal interactions of a panel of human recombinant Fabs derived from an HCV-infected patient. Three unrelated epitopes recognized by antibodies with no neutralization-of-binding (NOB) activity were identified; a fourth, major epitope was defined as a clustering of minor epitopes recognized by Fabs endowed with strong NOB activity.

Bugli, F., Mancini, N., Kang, C., Di Campli, C., Grieco, A., Manzin, A., Gabrielli, A., Gasbarrini, A., Fadda, G., Varaldo, P., Clementi, M. E., Burioni, R., Mapping B-cell epitopes of hepatitis C virus E2 glycoprotein using human monoclonal antibodies from phage display libraries, <<JOURNAL OF VIROLOGY>>, 2001; 75 (20): 9986-9990. [doi:10.1128/JVI.75.20.9986-9990.2001] [http://hdl.handle.net/10807/20513]

Mapping B-cell epitopes of hepatitis C virus E2 glycoprotein using human monoclonal antibodies from phage display libraries

Bugli, Francesca;Gasbarrini, Antonio;Fadda, Guido;Clementi, Maria Elisabetta;
2001

Abstract

Clinical and experimental evidence indicates that the hepatitis C virus (HCV) E2 glycoprotein (HCV/E2) is the most promising candidate for the development of an effective anti-HCV vaccine. Identification of the human epitopes that are conserved among isolates and are able to elicit protective antibodies would constitute a significant step forward. This work describes the mapping of the B-cell epitopes present on the surface of HCV/E2, as recognized by the immune system during infection, by the analysis of the reciprocal interactions of a panel of human recombinant Fabs derived from an HCV-infected patient. Three unrelated epitopes recognized by antibodies with no neutralization-of-binding (NOB) activity were identified; a fourth, major epitope was defined as a clustering of minor epitopes recognized by Fabs endowed with strong NOB activity.
2001
Inglese
Bugli, F., Mancini, N., Kang, C., Di Campli, C., Grieco, A., Manzin, A., Gabrielli, A., Gasbarrini, A., Fadda, G., Varaldo, P., Clementi, M. E., Burioni, R., Mapping B-cell epitopes of hepatitis C virus E2 glycoprotein using human monoclonal antibodies from phage display libraries, <<JOURNAL OF VIROLOGY>>, 2001; 75 (20): 9986-9990. [doi:10.1128/JVI.75.20.9986-9990.2001] [http://hdl.handle.net/10807/20513]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/20513
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