In this chapter, we provide a methodological description of the process to perform gastrointestinal (GIT) microbiota profiling on human stool samples. The process includes: (i) collection of feces, (ii) isolation of DNA from fecal community bacteria, (iii) selection of both 16S rDNA sequencing target and next-generation sequencing platform, and (iv) analysis and interpretation of sequence data. The process culminates into a comprehensive report on the GIT microbiota composition and structure that may translate into clinically actionable results.
Posteraro, B., De Maio, F., Gasbarrini, A., Profiling the Gastrointestinal Microbiota, <<Helicobacter Pylori>>, n/a; 2283 (N/A): 83-92. [doi:10.1007/978-1-0716-1302-3_10] [http://hdl.handle.net/10807/204183]
Profiling the Gastrointestinal Microbiota
Posteraro, Brunella;De Maio, Flavio;Gasbarrini, Antonio
2021
Abstract
In this chapter, we provide a methodological description of the process to perform gastrointestinal (GIT) microbiota profiling on human stool samples. The process includes: (i) collection of feces, (ii) isolation of DNA from fecal community bacteria, (iii) selection of both 16S rDNA sequencing target and next-generation sequencing platform, and (iv) analysis and interpretation of sequence data. The process culminates into a comprehensive report on the GIT microbiota composition and structure that may translate into clinically actionable results.
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