Background: Psoriasis and atopic dermatitis (AD) are common, complex inflammatory skin diseases. Both diseases display immune infiltrates in lesions and epidermal growth/differentiation alterations associated with a defective skin barrier. An incomplete understanding of differences between these diseases makes it difficult to compare human disease pathology to animal disease models. Objective: To characterize differences between these diseases in expression of genes related to epidermal growth/differentiation and inflammatory circuits. Methods: We performed genomic profiling of mRNA in chronic psoriasis (n = 15) and AD (n = 18) skin lesions compared with normal human skin (n = 15). Results: As expected, clear disease classifications could be constructed on the basis of expected immune polarity (TH1, TH2, TH17) differences. However, even more striking differences were identified in epidermal differentiation programs that could be used for precise disease classifications. Although both psoriasis and AD skin lesions displayed regenerative epidermal hyperplasia, which is a general alteration in epidermal growth, keratinocyte terminal differentiation was differentially polarized. In AD, we found selective defects in expression of multiple genes encoding the cornified envelope, with the largest alteration in loricrin (expressed at 2% of the level of normal skin). At the ultrastructural level, the cornified envelope in AD was broadly defective with highly decreased compaction of corneocytes and reduced intercellular lipids. Hence, the entire keratinocyte terminal differentiation program (cytoplasmic compaction, cornification, and lipid release) is defective in AD, potentially underlying the immune differences. Conclusion: Our study shows that although alterations in barrier responses exist in both diseases, epidermal differentiation is differentially polarized, with major implications for primary disease pathogenesis. © 2009 American Academy of Allergy, Asthma & Immunology.

Guttman-Yassky, E., Suarez-Farinas, M., Chiricozzi, A., Nograles, K. E., Shemer, A., Fuentes-Duculan, J., Cardinale, I., Lin, P., Bergman, R., Bowcock, A. M., Krueger, J. G., Broad defects in epidermal cornification in atopic dermatitis identified through genomic analysis, <<JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY>>, 2009; 124 (6): 1235-e58. [doi:10.1016/j.jaci.2009.09.031] [http://hdl.handle.net/10807/200291]

Broad defects in epidermal cornification in atopic dermatitis identified through genomic analysis

Chiricozzi, Andrea;
2009

Abstract

Background: Psoriasis and atopic dermatitis (AD) are common, complex inflammatory skin diseases. Both diseases display immune infiltrates in lesions and epidermal growth/differentiation alterations associated with a defective skin barrier. An incomplete understanding of differences between these diseases makes it difficult to compare human disease pathology to animal disease models. Objective: To characterize differences between these diseases in expression of genes related to epidermal growth/differentiation and inflammatory circuits. Methods: We performed genomic profiling of mRNA in chronic psoriasis (n = 15) and AD (n = 18) skin lesions compared with normal human skin (n = 15). Results: As expected, clear disease classifications could be constructed on the basis of expected immune polarity (TH1, TH2, TH17) differences. However, even more striking differences were identified in epidermal differentiation programs that could be used for precise disease classifications. Although both psoriasis and AD skin lesions displayed regenerative epidermal hyperplasia, which is a general alteration in epidermal growth, keratinocyte terminal differentiation was differentially polarized. In AD, we found selective defects in expression of multiple genes encoding the cornified envelope, with the largest alteration in loricrin (expressed at 2% of the level of normal skin). At the ultrastructural level, the cornified envelope in AD was broadly defective with highly decreased compaction of corneocytes and reduced intercellular lipids. Hence, the entire keratinocyte terminal differentiation program (cytoplasmic compaction, cornification, and lipid release) is defective in AD, potentially underlying the immune differences. Conclusion: Our study shows that although alterations in barrier responses exist in both diseases, epidermal differentiation is differentially polarized, with major implications for primary disease pathogenesis. © 2009 American Academy of Allergy, Asthma & Immunology.
2009
Inglese
Guttman-Yassky, E., Suarez-Farinas, M., Chiricozzi, A., Nograles, K. E., Shemer, A., Fuentes-Duculan, J., Cardinale, I., Lin, P., Bergman, R., Bowcock, A. M., Krueger, J. G., Broad defects in epidermal cornification in atopic dermatitis identified through genomic analysis, <<JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY>>, 2009; 124 (6): 1235-e58. [doi:10.1016/j.jaci.2009.09.031] [http://hdl.handle.net/10807/200291]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/200291
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