The present investigation aimed to characterize the protein profile of cavitating ultrasound aspirator fluid of newly diagnosed and recurrent glioblastoma comparing diverse zones of collection, i.e., tumor core and tumor periphery, with the aid of 5‐aminolevulinic acid fluorescence. The samples were pooled and analyzed in triplicate by LC‐MS following the shotgun proteomic approach. The identified proteins were then grouped to disclose elements exclusive and common to the tumor state or tumor zones and submitted to gene ontology classification and pathway overrepresentation analysis. The proteins common to the distinct zones were further investigated by relative quantitation, following a label free approach, to disclose possible differences of expression. Nine proteins, i.e., tubulin 2B chain, CD59, far upstream element‐binding, CD44, histone H1.4, caldesmon, osteopontin, tropomyosin chain and metallothionein‐2, marked the core of newly diagnosed glioblastoma with respect to tumor periphery. Considering the tumor zone, including the core and the fluorescence positive periphery, the serine glycine biosynthesis, pentose phosphate, 5‐ hydroxytryptamine degredation, de novo purine biosynthesis and huntington disease pathways resulted statistically significantly overrepresented with respect to the human genome of reference. The fluorescence negative zone shared several protein elements with the tumor zone, possibly indicating the presence of pathological aspects of glioblastoma rather than of normal brain parenchyma. On the other hand, its exclusive protein elements were considered to represent the healthy zone and, accordingly, exhibiting no pathways overrepresentation. On the contrary to newly diagnosed glioblastoma, pathway overrepresentation was recognized only in the healthy zone of recurrent glioblastoma. The TGFβ signaling pathway, exclusively classified in the fluorescence negative periphery in newly diagnosed glioblastoma, was instead the exclusive pathway classified in the tumor core of recurrent glioblastoma. These results, preliminary obtained on sample pools, demonstrated the potential of cavitron ultrasonic sur gical aspirate fluid for proteomic profiling of glioblastoma able to distinguish molecular features specific of the diverse tumor zones and tumor states, possibly contributing to the understanding of the highly infiltrative capability and recurrent rate of this aggressive brain tumor and opening to potential clinical applications to be further investigated.

La Rocca, G., Simboli, G. A., Vincenzoni, F., Rossetti, D. V., Urbani, A., Ius, T., Della Pepa, G. M., Olivi, A., Sabatino, G., Desiderio, C., Glioblastoma cusa fluid protein profiling: A comparative investigation of the core and peripheral tumor zones, <<CANCERS>>, 2021; 13 (1): 1-29. [doi:10.3390/cancers13010030] [http://hdl.handle.net/10807/176510]

Glioblastoma cusa fluid protein profiling: A comparative investigation of the core and peripheral tumor zones

La Rocca, Giuseppe
Primo
Writing – Original Draft Preparation
;
Rossetti, Diana Valeria;Urbani, Andrea;Della Pepa, Giuseppe Maria;Olivi, Alessandro;Sabatino, Giovanni
Penultimo
Supervision
;
Desiderio, Claudia
Ultimo
Writing – Review & Editing
2021

Abstract

The present investigation aimed to characterize the protein profile of cavitating ultrasound aspirator fluid of newly diagnosed and recurrent glioblastoma comparing diverse zones of collection, i.e., tumor core and tumor periphery, with the aid of 5‐aminolevulinic acid fluorescence. The samples were pooled and analyzed in triplicate by LC‐MS following the shotgun proteomic approach. The identified proteins were then grouped to disclose elements exclusive and common to the tumor state or tumor zones and submitted to gene ontology classification and pathway overrepresentation analysis. The proteins common to the distinct zones were further investigated by relative quantitation, following a label free approach, to disclose possible differences of expression. Nine proteins, i.e., tubulin 2B chain, CD59, far upstream element‐binding, CD44, histone H1.4, caldesmon, osteopontin, tropomyosin chain and metallothionein‐2, marked the core of newly diagnosed glioblastoma with respect to tumor periphery. Considering the tumor zone, including the core and the fluorescence positive periphery, the serine glycine biosynthesis, pentose phosphate, 5‐ hydroxytryptamine degredation, de novo purine biosynthesis and huntington disease pathways resulted statistically significantly overrepresented with respect to the human genome of reference. The fluorescence negative zone shared several protein elements with the tumor zone, possibly indicating the presence of pathological aspects of glioblastoma rather than of normal brain parenchyma. On the other hand, its exclusive protein elements were considered to represent the healthy zone and, accordingly, exhibiting no pathways overrepresentation. On the contrary to newly diagnosed glioblastoma, pathway overrepresentation was recognized only in the healthy zone of recurrent glioblastoma. The TGFβ signaling pathway, exclusively classified in the fluorescence negative periphery in newly diagnosed glioblastoma, was instead the exclusive pathway classified in the tumor core of recurrent glioblastoma. These results, preliminary obtained on sample pools, demonstrated the potential of cavitron ultrasonic sur gical aspirate fluid for proteomic profiling of glioblastoma able to distinguish molecular features specific of the diverse tumor zones and tumor states, possibly contributing to the understanding of the highly infiltrative capability and recurrent rate of this aggressive brain tumor and opening to potential clinical applications to be further investigated.
2021
Inglese
La Rocca, G., Simboli, G. A., Vincenzoni, F., Rossetti, D. V., Urbani, A., Ius, T., Della Pepa, G. M., Olivi, A., Sabatino, G., Desiderio, C., Glioblastoma cusa fluid protein profiling: A comparative investigation of the core and peripheral tumor zones, <<CANCERS>>, 2021; 13 (1): 1-29. [doi:10.3390/cancers13010030] [http://hdl.handle.net/10807/176510]
File in questo prodotto:
File Dimensione Formato  
Desiderio cancers.pdf

accesso aperto

Tipologia file ?: Versione Editoriale (PDF)
Licenza: Creative commons
Dimensione 6.29 MB
Formato Adobe PDF
6.29 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/176510
Citazioni
  • ???jsp.display-item.citation.pmc??? 4
  • Scopus 6
  • ???jsp.display-item.citation.isi??? 6
social impact