Heterochromation and rDNA localization in the holocentric chromosomes of the aphid Aphis fabae has been investigated at a cytological level after C-banding, Giemsa, DAPI NOR staining.C-banding technique showed that heterochromatic areas result mainly clustered on the X chromosomes but, contrary to what observed in other aphid species, in A. fabae C-positive bands are clearly distinguishable also on each autosomal chromosome pair, thus allowing the identification of homologues and a reliable reconstruction of the karyotype. Moreover, silver staining localized rDNA genes on one telomere of each X chromosome; these are the only brightly fluorescent C-positive regions revealed after CMA3 staining, whereas all other heterochromatic bands are DAPI positive. Both CMA3 and silver staining evidenced a noticeable amount of rDNA heteromorphism

Rivi, M., Cassanelli, S., Mazzoni, E., Bizzaro, D., Manicardi, G. C., Heterochromatin and rDNA localization on the holocentric chromosomes of black bean aphid, Aphis fabae Scop. (Hemiptera: Aphididae), <<CARYOLOGIA>>, 2009; 62 (4): 341-346 [http://hdl.handle.net/10807/17464]

Heterochromatin and rDNA localization on the holocentric chromosomes of black bean aphid, Aphis fabae Scop. (Hemiptera: Aphididae)

Mazzoni, Emanuele;
2009

Abstract

Heterochromation and rDNA localization in the holocentric chromosomes of the aphid Aphis fabae has been investigated at a cytological level after C-banding, Giemsa, DAPI NOR staining.C-banding technique showed that heterochromatic areas result mainly clustered on the X chromosomes but, contrary to what observed in other aphid species, in A. fabae C-positive bands are clearly distinguishable also on each autosomal chromosome pair, thus allowing the identification of homologues and a reliable reconstruction of the karyotype. Moreover, silver staining localized rDNA genes on one telomere of each X chromosome; these are the only brightly fluorescent C-positive regions revealed after CMA3 staining, whereas all other heterochromatic bands are DAPI positive. Both CMA3 and silver staining evidenced a noticeable amount of rDNA heteromorphism
Inglese
Rivi, M., Cassanelli, S., Mazzoni, E., Bizzaro, D., Manicardi, G. C., Heterochromatin and rDNA localization on the holocentric chromosomes of black bean aphid, Aphis fabae Scop. (Hemiptera: Aphididae), <<CARYOLOGIA>>, 2009; 62 (4): 341-346 [http://hdl.handle.net/10807/17464]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10807/17464
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