We developed a new method based on the Nanochip microelectronic array technology for identification of various clinically relevant mycobacterial species. PCR-amplified rRNA genes obtained from 270 positive Mycobacteria Growth Indicator Tube cultures were successfully tested by hybridizing them with species-selective probes, and the results agreed with those of conventional identification methods. The system is rapid and accurate and opens new perspectives in clinical diagnostics.
Sanguinetti, M., Novarese, L., Posteraro, B., Ranno, S., De Carolis, E., Pecorini, G., Lucignano, B., Ardito, F., Fadda, G., Use of microelectronic array technology for rapid identification of clinically relevant mycobacteria, <<JOURNAL OF CLINICAL MICROBIOLOGY>>, 2005; 43 (12): 6189-6193. [doi:10.1128/JCM.43.12.6189-6193.2005] [http://hdl.handle.net/10807/17410]
Use of microelectronic array technology for rapid identification of clinically relevant mycobacteria
Sanguinetti, Maurizio;Novarese, Linda;Posteraro, Brunella;Ranno, Stefania;De Carolis, Elena;Pecorini, Giovanni;Lucignano, Barbara;Ardito, Fausta;Fadda, Giovanni
2005
Abstract
We developed a new method based on the Nanochip microelectronic array technology for identification of various clinically relevant mycobacterial species. PCR-amplified rRNA genes obtained from 270 positive Mycobacteria Growth Indicator Tube cultures were successfully tested by hybridizing them with species-selective probes, and the results agreed with those of conventional identification methods. The system is rapid and accurate and opens new perspectives in clinical diagnostics.
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