The environment, including animals and animal products, is colonized by bacterial species that are typical and specific of every different ecological niche. Natural and human-related ecological pressure promotes the selection and expression of genes related to antimicrobial resistance (AMR). These genes might be present in a bacterial consortium but might not necessarily be expressed. Their expression could be induced by the presence of antimicrobial compounds that could originate from a given ecological niche or from human activity. In this work, we applied (meta)proteomics analysis of bacterial compartment of raw milk in order to obtain a method that provides a measurement of circulating AMR involved proteins and gathers information about the whole bacterial composition. Results from milk analysis revealed the presence of 29 proteins/proteoforms linked to AMR. The detection of mainly β-lactamases suggests the possibility of using the milk microbiome as a bioindicator for the investigation of AMR. Moreover, it was possible to achieve a culture-free qualitative and functional analysis of raw milk bacterial consortia.

Piras, C., Greco, V., Gugliandolo, E., Soggiu, A., Tilocca, B., Bonizzi, L., Zecconi, A., Cramer, R., Britti, D., Urbani, A., Roncada, P., Raw cow milk bacterial consortium as bioindicator of circulating anti-microbial resistance (Amr), <<ANIMALS>>, 2020; 10 (12): 1-13. [doi:10.3390/ani10122378] [http://hdl.handle.net/10807/167535]

Raw cow milk bacterial consortium as bioindicator of circulating anti-microbial resistance (Amr)

C.; Greco;D.; Urbani;
2020

Abstract

The environment, including animals and animal products, is colonized by bacterial species that are typical and specific of every different ecological niche. Natural and human-related ecological pressure promotes the selection and expression of genes related to antimicrobial resistance (AMR). These genes might be present in a bacterial consortium but might not necessarily be expressed. Their expression could be induced by the presence of antimicrobial compounds that could originate from a given ecological niche or from human activity. In this work, we applied (meta)proteomics analysis of bacterial compartment of raw milk in order to obtain a method that provides a measurement of circulating AMR involved proteins and gathers information about the whole bacterial composition. Results from milk analysis revealed the presence of 29 proteins/proteoforms linked to AMR. The detection of mainly β-lactamases suggests the possibility of using the milk microbiome as a bioindicator for the investigation of AMR. Moreover, it was possible to achieve a culture-free qualitative and functional analysis of raw milk bacterial consortia.
Inglese
Piras, C., Greco, V., Gugliandolo, E., Soggiu, A., Tilocca, B., Bonizzi, L., Zecconi, A., Cramer, R., Britti, D., Urbani, A., Roncada, P., Raw cow milk bacterial consortium as bioindicator of circulating anti-microbial resistance (Amr), <<ANIMALS>>, 2020; 10 (12): 1-13. [doi:10.3390/ani10122378] [http://hdl.handle.net/10807/167535]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/167535
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