Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based species identification has found its place in many clinical routine diagnostic laboratories over the past years, allowing significantly reduced turnaround times and high-precision results. With regard to MALDI-TOF MS for filamentous fungi, here, we discuss different approaches for sample processing and growth conditions before analysis. In particular, we review the performances of different commercially available databases as well as the potential of complementary (self-constructed) in-house databases.

Wilkendorf, L. S., Bowles, E., Buil, J. B., Van Der Lee, H. A. L., Posteraro, B., Sanguinetti, M., Verweij, P. E., Update on matrix-assisted laser desorption ionization-time of flight mass spectrometry identification of filamentous fungi, <<JOURNAL OF CLINICAL MICROBIOLOGY>>, 2020; 58 (12): 1-9. [doi:10.1128/JCM.01263-20] [http://hdl.handle.net/10807/166927]

Update on matrix-assisted laser desorption ionization-time of flight mass spectrometry identification of filamentous fungi

Posteraro, Brunella;Sanguinetti, Maurizio;
2020

Abstract

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based species identification has found its place in many clinical routine diagnostic laboratories over the past years, allowing significantly reduced turnaround times and high-precision results. With regard to MALDI-TOF MS for filamentous fungi, here, we discuss different approaches for sample processing and growth conditions before analysis. In particular, we review the performances of different commercially available databases as well as the potential of complementary (self-constructed) in-house databases.
2020
Inglese
Wilkendorf, L. S., Bowles, E., Buil, J. B., Van Der Lee, H. A. L., Posteraro, B., Sanguinetti, M., Verweij, P. E., Update on matrix-assisted laser desorption ionization-time of flight mass spectrometry identification of filamentous fungi, <<JOURNAL OF CLINICAL MICROBIOLOGY>>, 2020; 58 (12): 1-9. [doi:10.1128/JCM.01263-20] [http://hdl.handle.net/10807/166927]
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/166927
Citazioni
  • ???jsp.display-item.citation.pmc??? 10
  • Scopus 26
  • ???jsp.display-item.citation.isi??? 26
social impact